Based on our complete Carbohydrate Metabolism Analysis solutions, CD BioGlyco provides high-quality sucrose analysis services in fruits, grains, vegetables, plants, and more by various methods.
Sucrose, also known as α-D-glucopyranosyl-β-D-fructofuranoside, is a non-reducing sugar composed of glucose and fructose subunits, which is the highest production of any single, pure, natural, organic chemical in the world. It is mainly found in oxygenated photosynthetic organisms, including plants, unicellular algae, and cyanobacteria. Sucrose is a convenient form of carbon transport because of its unusual linkage between the anomeric C-1 position of glucose and the anomeric C-2 position of fructose. Therefore, sucrose is used as a transport and storage molecule in most plants. At present, sucrose has been widely used in biscuits, chocolate, jams, and other food industries.
Fig.1 Sucrose structure. (Wikipedia)
First, dihydroxyacetone phosphate and glyceraldehyde 3-phosphate are catalyzed by aldolase to form fructose 1,6-bisphosphate. The hydrolysis of fructose 1,6-diphosphate forms fructose-6-phosphate. On the other hand, glucose-1-phosphate and uridine-5′-triphosphate (UTP) are catalyzed by uridine diphosphate (UDP)-glucose pyrophosphorylase to generate UDP-glucose. Then, sucrose-6-phosphate synthase catalyzes fructose-6-phosphate and UDP-glucose to form sucrose-6-phosphate. Finally, sucrose-6-phosphate removes the phosphate group under the action of sucrose phosphatase to form sucrose. In addition, both sucrose synthase (SuS) and invertase can hydrolyze sucrose to produce glucose and fructose.
Fig.2 Sucrose metabolism in higher plants. (Salerno, 2003)
As the main product of photosynthesis, sucrose is involved in plant growth, development, storage, and signal transduction. At CD BioGlyco, we have developed complete Carbohydrate Metabolism Analysis solutions, providing sucrose analysis services in fruits, grains, vegetables, and plants. Our methods include:
HPLC is the most common chromatographic technique for the determination of sugar content. We can determine sucrose content in fruits, grains, vegetables, and plants by HPLC with refractive index detection (HPLC-RID), HPLC with evaporative light scattering detector (HPLC-ELSD), and HPLC-mass spectrometry (HPLC-MS).
HPAEC-PAD has higher sensitivity and is suitable for the determination of sucrose content in low-level samples.
Vis-NIR spectroscopy is used to predict sucrose content in sugar beets.
CD BioGlyco is a leader in carbohydrate research, providing accurate, reliable, and reproducible sucrose analysis services for our global clients. If you are interested in our services, please feel free to contact us, we are looking forward to being your research assistant in the field of carbohydrates.