CD BioGlyco has rich experience in lectin microarray assay. Our multiple technology platforms and well-trained researchers can provide customers with various forms of sample testing. We have confidence to be your essential research assistant in the field of glycobiology.

Background

Lectin is a glycoprotein that can bind to sugar, be synthesized secreted by animal cells and plant cells. Lectins can recognize complex carbohydrate structures in glycoproteins and glycolipids, as well as sugar groups on the surface of cell membranes. Therefore, lectin is a useful tool for studying cell membrane structures, cell recognition and adhesion reactions. In addition, because of its multivalent binding ability, lectin can bind to luciferin, enzymes, ferritin, biotin, etc. without affecting its biological activity. Therefore, lectin can also be used in immunocytochemistry research and tumor diagnosis. Start a new paragraph: Lectin microarray is a new method of glycan profile analysis based on lectin. It has become a very promising method in the field of glycomics and glycoproteomics. It uses a set of lectins fixed on a solid surface to perform rapid analysis of glycoprotein glycosylation profile and glycoform characterization, and can be directly applied to both pure and crude form (for example, clinical samples) of glycoprotein. Unlike other conventional methods (such as liquid chromatography and mass spectrometry), it can analyze complex glycan characteristics quickly and with high sensitivity without releasing glycans. Although this technique is not quantitative, it's more efficiently applied for comparative purposes, such as differential profiling.

Fig. 1 Lectin and antibody microarrays are used for glycan analysis and bacterial detection. (Campanero-Rhodes, et al., 2020)

Services

CD BioGlyco uses the following methods to perform lectin microarray assay.

We provide professional lectin microarray system, including equipment, microarray plate and various detection technologies (Confocal fluorescence detection method/ Evanescent-field activated fluorescence detection method/ Bimolecular fluorescence quenching and recovery method). Subsequently, we develop a set of advanced and general procedure to prepare samples which can be used for various forms of samples such as serum, urine, cell lysates and tissue extracts. Professional technicians will perform fluorescent labeling and data analysis and finally draw a precise conclusion.

Our microarrays contain multiple lectins for glycan analysis. All lectins have been validated to ensure their binding specificity. By fluorescently labeling samples, incubating them with the microarray, and quantitatively detecting fluorescent interactions, we analyze the glycome of the samples. We deliver histograms, data files, and detailed result discussions. Additionally, lectin microarrays can be customized to meet various personalized needs and tested and analyzed according to specific requirements. Whether your research objectives involve disease biomarker discovery, drug development, or fundamental glycobiology exploration, our lectin chip services provide robust data support.

Workflow

Our lectin microarray service follows strict quality control standards to ensure data accuracy and reliability, including the following:

• Sample reception: We receive the samples and perform the necessary sample preparation and protein separation steps. We then label the samples with fluorescent dyes and clean them.
• Lectin microarray incubation: The processed samples are incubated with our pre-prepared high-quality lectin chips, and conditions are optimized to ensure that target molecules bind to the corresponding lectins.
• Binding analysis: Fluorescent signals are scanned using a high-resolution microarray scanner, and image data is quantitatively analyzed using specialized software.
• Data processing: We standardize the raw data and perform lectin interaction analysis, and complete a detailed detection report. The binding data is provided in the form of a histogram. The report also includes the raw data and fluorescent images of the lectin microarray after incubation.

Applications

• Glycan-related biomarker research: By analyzing the glycosylation characteristics of inflammatory diseases, neurodegenerative diseases, tumors, etc., to promote the discovery of disease-related biomarkers.
• Drug development: Utilizing lectin microarrays for glycosylation analysis of biopharmaceuticals can provide data support for analyzing the impact of glycosylation on drug efficacy, safety, and immunogenicity, as well as evaluating drug production processes.
• Glycoprotein analysis under functional glycomics: Analysis using lectin microarrays helps understand the role of glycoproteins in cell-to-cell communication, adhesion, migration, signaling pathways, etc.

Advantages

• Our lectin microarray is well-suited for the simultaneous analysis of multiple samples, providing rapid results in a simple and user-friendly format.
• Our lectin microarray is suitable for the analysis of various biological samples, such as proteins, antibodies, cells, cell lysates, serum, bacteria, etc.
• The assay has high sensitivity and specificity and is suitable for detecting minute glycosylation changes in low-abundance glycoproteins.

Publication Data

Frequently Asked Questions

CD BioGlyco has provided reliable and cost-effective research services to customers all over the world, and our considerate service and professional technology in the field of glycobiology have won us a great deal of praise.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

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