Glycobiology Microarray Platform

Microarray is a two-dimensional arrangement of specific biological probes (such as DNA, proteins, polysaccharides, and products or libraries of low-molecular-weight natural compounds), arranged in an addressable manner on glass slides or other substrates (such as polymer-coated glass, nitrocellulose, or plastic). It is a platform for parallel analysis of complex biological liquids in a tiny grid-like plane. Thousands of isolated biological probes with diameters ranging from 50 to 300 µm are usually arranged on the surface of the glass slide, and each spot in the array can represent a biochemical event and can be measured independently. This component allows for high-throughput multiplex analysis, usually combined with fluorescence to detect many different analytes simultaneously.

In addition to DNA and protein microarrays, there are also cell and tissue microarrays. The array combines mature cell research methods with the high-throughput screening function of microarrays, and can be used for drug screening, in vitro toxicology testing, and functional genomics research. Today, microarrays have been widely used in genome research and can be applied to various applications in the fields of biology and medicine. Microarrays accelerate the process of understanding the function of genes, proteins, and cells in living organisms. They can also be used as molecular markers for disease diagnosis, prediction, and prevention.

Fig.1 Construction of cell-based glycan arrays. (Briard, et al., 2018)

Our Services

We provide comprehensive glycobiology microarray-based assay services covering all aspects from experimental design to data analysis to meet diverse research needs.

Customized Glycobiology Microarray Design and Construction

We have a wide range of glycobiology libraries, including natural oligosaccharides, synthetic glycan chains, glycoproteins, glycolipids, and so on. Our researchers customize the design and construction of the most suitable glycobiology microarray according to the specific research objectives and biomolecular properties, with a wide range of substrates to choose from and flexible sampling.

Sample Preparation

Our glycobiology microarray platform is suitable for a wide range of biological sample types (proteins, antibodies, cells, tissues, etc.). Combined with extensive experimental experience, samples are processed and labeled upon receipt.

Hybridization and Washing

We utilize glycobiology microarrays to detect biomolecules (glycoproteins, glycolipids, lectins, antibodies, etc.) for hybridization and cleaning. State-of-the-art equipment ensures precise control and efficient execution of the entire process.

• Glycoprotein binding analysis: We utilize glycobiology microarrays to study the interactions between proteins and specific glycans. We reveal the role of glycans in protein function and discover new glycan-binding proteins.
• Antibody specificity analysis: Glycobiology microarray is used to evaluate the specific binding ability of antibodies to glycans and to validate the targeting and reliability of antibodies.
• Other types of analysis, including but not limited to transcript expression analysis.

Data Analysis

We use professional analysis software for data extraction and processing, and mine valuable biological information from complex microarray data, including but not limited to differential binding analysis, clustering analysis, and GO enrichment analysis.

Workflow

Advantages

• Our glycobiology microarray supports high-throughput analysis. Only a small amount of sample is needed to screen for multiple interactions simultaneously, speeding up the research process.
• Our glycobiology microarray is versatile and can be used to analyze a wide range of types, such as lectins, glycoproteins, and more.
• Our glycobiology microarrays are optimized for high sensitivity and specificity.

Applications

• These glycobiology microarrays are used to identify specific proteins that can bind to glycans to analyze the role of these glycans in various biological processes.
• These glycobiology microarrays are used to analyze the binding specificity of glycan-binding proteins, including preferred glycan structures, linkage types, and more.
• In disease studies, these glycobiology microarrays are used to understand the interactions mediated by glycans, which are essential for screening potential drug targets and related lead compounds.

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Frequently Asked Questions

CD BioGlyco provides different kinds of comprehensive microarray analysis services, which not only achieve high throughput and high accuracy but also save costs and time for clients. We hope to start the exploration of glycobiology with you. If you are interested in our services, please contact us for more detailed information.

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