Glycobiology Microarray Platform
Microarray is a two-dimensional arrangement of specific biological probes (such as DNA, proteins, polysaccharides, and products or libraries of low-molecular-weight natural compounds), arranged in an addressable manner on glass slides or other substrates (such as polymer-coated glass, nitrocellulose, or plastic). It is a platform for parallel analysis of complex biological liquids in a tiny grid-like plane. Thousands of isolated biological probes with diameters ranging from 50 to 300 µm are usually arranged on the surface of the glass slide, and each spot in the array can represent a biochemical event and can be measured independently. This component allows for high-throughput multiplex analysis, usually combined with fluorescence to detect many different analytes simultaneously.
In addition to DNA and protein microarrays, there are also cell and tissue microarrays. The array combines mature cell research methods with the high-throughput screening function of microarrays, and can be used for drug screening, in vitro toxicology testing, and functional genomics research. Today, microarrays have been widely used in genome research and can be applied to various applications in the fields of biology and medicine. Microarrays accelerate the process of understanding the function of genes, proteins, and cells in living organisms. They can also be used as molecular markers for disease diagnosis, prediction, and prevention.
Fig.1 Construction of cell-based glycan arrays. (Briard, et al., 2018)
We provide comprehensive glycobiology microarray-based assay services covering all aspects from experimental design to data analysis to meet diverse research needs.
We have a wide range of glycobiology libraries, including natural oligosaccharides, synthetic glycan chains, glycoproteins, glycolipids, and so on. Our researchers customize the design and construction of the most suitable glycobiology microarray according to the specific research objectives and biomolecular properties, with a wide range of substrates to choose from and flexible sampling.
Our glycobiology microarray platform is suitable for a wide range of biological sample types (proteins, antibodies, cells, tissues, etc.). Combined with extensive experimental experience, samples are processed and labeled upon receipt.
We utilize glycobiology microarrays to detect biomolecules (glycoproteins, glycolipids, lectins, antibodies, etc.) for hybridization and cleaning. State-of-the-art equipment ensures precise control and efficient execution of the entire process.
We use professional analysis software for data extraction and processing, and mine valuable biological information from complex microarray data, including but not limited to differential binding analysis, clustering analysis, and GO enrichment analysis.
Compared with traditional methods of detecting the interaction of glycans and GBP, glycan microarray has the advantages of rapidity, accuracy, and cost savings. Glycan microarrays include the following:
We developed microassay techniques for providing structural information on glycosphingolipid, which plays an important role in numerous biological processes.
We have developed heparin sulfate microarrays to help clients accelerate their scientific research. One-stop service makes clients more worry-free.
The microarrays we have established have accelerated progress in the study of the function and interactions of breast milk oligosaccharides.
Lectin can be used as a useful tool for studying cell recognition and adhesion. We provide a fast and high-throughput lectin microarray assay, which performs well in differential analysis.
Our glyco-gene microarray assay is an advanced technology for screening and detecting changes in the transcription and expression levels of glycan-related genes.
In addition to being used as biomarkers, glycoprotein microarray can also be used to screen various vaccines, so it has received widespread attention.
We have collaborated with several research teams and provided them with glycopeptide microarray services to help them screen antibodies.
CD BioGlyco tailors the microarray printing and analysis experiments according to the client's sample type and project needs. All you need to do is to make a request.
CD BioGlyco provides high-quality microarray-based glycobiological analysis solutions for our global clients.
Technology: Glycan microarray
Journal: Frontiers in Chemistry
IF: 3.693
Published: 2019
Results: Glycan microarrays are useful tools for studying glycan-glycan binding protein (GBP) interactions. This paper introduces us to strategies for the construction of glycan microarrays and their immunological applications, with a focus on the types of microarrays that contain the most comprehensive glycan structures. One strategy for constructing glycan microarrays is covalent linkage, i.e., covalent derivatization of a glycan with a specific bifunctional chemical moiety, which then covalently reacts with the functionalized solid surface. A variety of linkers have been developed to label glycans and generate microarrays. These constructed microarrays can be used to recognize endogenous immunoreceptors and other immunological applications.
Fig.2 A bifunctional fluorescent glycan junction. (Gao, et al., 2019)
CD BioGlyco provides different kinds of comprehensive microarray analysis services, which not only achieve high throughput and high accuracy but also save costs and time for clients. We hope to start the exploration of glycobiology with you. If you are interested in our services, please contact us for more detailed information.
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