AXOS is a pentose oligomer derived from arabinoxylans (AX) by physicochemical or enzymatic treatments. AX degrades to AXOS by enzymatic hydrolysis in the colon using intestinal microbiota or during food processing. AXOS belongs to dietary fibers. Studies have shown the prebiotic effects of AXOS in the colons of humans and animals. AX and AXOS are associated with many health benefits, including immunomodulatory activity and attenuation of type II diabetes. AXOS has also been reported to attenuate serum cholesterol, triglyceride, and glucose levels.
Fig.1 A summary of the biological activities of AXOS. (Chen, et al., 2019)
With years of experience, CD BioGlyco provides customized approaches combining the appropriate preparation and analysis techniques to study oligosaccharides. We offer all types of Oligosaccharide Analytical Services. We provide customized analytical studies using our large range of analytical techniques implemented by our skilled analytical team.
HPLC is at present the main analytical technique used for carbohydrates analysis.
RP-HPLC is applied for the structural characterization of derivatized AXOS.
MS/TMS is applied for the determination of linkage positions and sequences of oligosaccharides.
PGC-HILIC has enabled the HPLC-MS or HPLC-MS/MS analysis of oligosaccharides without derivatization.
Applied for determination of the molecular weight distribution of AXOS.
Applied for identification of the structure elucidation of AXOS.
HPAEC-PAD is used to analyze quantitatively after being
For example, we identify cereal AXOS by negative ionization HILIC-MS/MS and perform structural analysis. The analysis process is as follows:
1) Sample preparation and extraction: AXOS is prepared from wheat and oats by using different hydrolyzing enzyme treatments.a
2) Analysis: Hydrolysates are fractionated by GPC, separated by HPLC, and identified by HPAEC-PAD and NMR spectroscopy.
3) Structural analysis is performed by negative ionization HILIC-MS/MS.
Fig.2 AXOS analytical techniques. (CD BioGlyco)
CD BioGlyco has provided high-quality AXOS analysis services over the years and has been a rich experience. If you need our services, please feel free to contact us.