Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is one of the most sensitive genetic analysis techniques available. It has a wide range of applications, including quantitative gene expression analysis, genotyping, drug target validation, biomarker discovery, pathogen detection, and measuring RNA interference. Intensive quality control is a vital part of qRT-PCR that can be captured with plan/prepare, cycle, and report.
RT-qPCR can be done by a one- or two-step method, the difference between which is whether the reverse transcription step and the PCR amplification step are combined.
Fig.1 Schematic diagram of one- or two-step RT-qPCR. (CD BioGlyco)
As a discovery tool, glycogene expression profiling explains the complex molecular background within organisms and provides excellent insight into new therapies for glycogene-related diseases. CD BioGlyco has comprehensive Platforms to help clients solve various challenges in the field of glycobiology. The workflow of qRT-PCR-based glycogene expression profiling at CD BioGlyco is as follows:
Fig.2 The workflow of qRT-PCR-based glycogene expression profiling. (CD BioGlyco)
With our qRT-PCR-based glycogene expression profiling service, you achieve any goal:
CD BioGlyco provides first-class qRT-PCR-based glycogene expression profiling. We analyze many RNA samples in parallel and offer accurate predictive data for you to ensure the smooth progress of your research. If you are interested in our services, please contact us in time, and we look forward to communicating with you.