Analysis of TCA Cycle Related Enzymes in Cancer Cells

Analysis of TCA Cycle Related Enzymes in Cancer Cells

Glucose is an important nutrient that supports the energy and biomacromolecules needed for the rapid proliferation of cancer cells. Glucose generates ATP through the tricarboxylic acid cycle, a precursor to nucleotides, amino acids, and fatty acids. The TCA cycle occurs in the mitochondria, and its metabolites are involved in many metabolic pathways within the cell. The TCA cycle pathway involves a variety of enzymes. The expression and activity of TCA cycle-related enzymes determine the rate of the TCA cycle. Loss or dysfunction of TCA cycle-related enzymes play different roles in the reprogramming of cancer glucose metabolism in regulating cancer progression. CD BioGlyco can provide TCA cycle-related enzymes expression detection, activity analysis, and analysis of the interactions between enzymes and proteins to further understand their role in cancer progression.

What We Do

  • Analysis of TCA cycle related enzymes activity

We can provide iTRAQ quantitative proteomics analysis based on cancer glucose metabolism, which can simultaneously analyze the expression of TCA cycle-related enzymes. The enzymes we are able to provide specific quantitative proteomic analysis are as follows, including but not limited to:

Citrate synthase
Isocitrate dehydrogenase
α-ketoglutarate dehydrogenase
Succinyl-CoA synthetase
Succinate dehydrogenase
Fumarate hydratase
Malate dehydrogenase

Fig. 1 TCA cycleFig. 1 TCA cycle (Choi I, et al., 2021)

  • Analysis of binary interactions between the TCA cycle related enzymes

Protein-protein interactions have multiple biological functions. In the regulation of glucose metabolism, the interaction between TCA cycle-related enzymes is of great significance. We are able to provide an analysis of TCA cycle-related enzyme interactions in vivo and in vitro.

  • Analysis of the role of TCA cycle metabolons

TCA cycle related enzymes can interact to form metabolic substructures, also known as metabolons, which play a regulatory role in the process of glucose metabolism. We can provide an analysis of the role of the TCA cycle metabolon.


Our service can support the following applications, including but not limited to:

  • Searching for novel mechanisms of cancer metabolic regulation through epigenetic modification of TCA cycle related enzymes.
  • Analyzing TCA cycle metabolic proteomic changes in cancer cells in response to metabolic modulators.
  • To explore the effect of dietary regulation on cancer progression by modulating the TCA cycle.
  • To explore the effects of cancer cell growth conditions on metabolic pathways and metabolic pathway-related protein profiles.

CD BioGlyco can provide quantitative detection services of cancer TCA cycle related enzymes, and TCA cycle related enzyme interaction analysis services. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.


  1. Choi I, et al. Tricarboxylic Acid (TCA) Cycle Intermediates: Regulators of Immune Responses. Life (Basel). 2021 Jan 19; 11(1): 69.
This service is for Research Use Only, not intended for any clinical use.

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