Short-chain Fatty Acid Analysis

Short-chain Fatty Acid Analysis

Short-chain fatty acids (SCFAs) are bacterial metabolites produced by components of the microbiome. Gas chromatography (GC) is the most common method to analyze fatty acids (FAs). CD BioGlyco is the leader in the field of Fatty Acid Analysis. Our scientists have been committed to the research of FAs analysis and accumulated rich experience. We provide customers with analysis services of a variety of SCFAs by GC.

Overview of SCFAs

SCFAs are organic fatty acids with 1-6 carbon atoms. The main production site of SCFAs is in the colon, which is mostly produced by anaerobic fermentation of carbohydrates in undigested and absorbed food residues, mainly including acetic acid, propionic acid, and butyric acid. SCFAs participate in different metabolisms and perform different functions in the human body. Among them, acetic acid is an important source of energy for host cells; Propionic acid enters the liver through blood circulation, is catabolized in the liver, and participates in the process of reverse conversion of pyruvate to glucose; Butyric acid is the main energy source for epithelial cells.

SCFAs exhibit important physiological functions, which reduce intestinal inflammation, improve intestinal epithelial barrier function, and inhibit colon tumors, non-alcoholic fatty liver disease, obesity, and other diseases. In short, it plays an irreplaceable role in maintaining body homeostasis and normal physiological metabolism. Therefore, appropriate analytical techniques are essential for the sensitive and accurate quantification of SCFAs.

Metabolism of SCFAs from dietary fiber to systemic circulation.Fig.1 Metabolism of SCFAs from dietary fiber to the systemic circulation. (Dalile, 2019)

Our Services

SCFAs are produced by fermentation of resistant starch and dietary fiber from the gut microbiota. The most important SCFAs are acetic acid (C2), propionic acid (C3), and butyric acid (C4). CD BioGlyco provides acetic acid, propionic acid, isobutyric acid, butyric acid, 2-methylbutyric acid, isovaleric acid, valeric acid, 2-methylvaleric acid, caproic acid, isocaproic acid, 3-methylvaleric acid, and 3-hydroxyiso valeric acid analysis services. We use GC to quantify SCFAs. Quantify SCFAs in the serum, colonic luminal content, and feces of mice by GC: 1) Sample preparation; 2) Sample analysis; 3) Data analysis.

Quantify SCFAs in the serum, colonic luminal content and feces of mice by GC.Fig.2 Quantify SCFAs in the serum, colonic luminal content and feces of mice by GC. (Ribeiro, 2018)


  • Disease marker screening
  • Exploration of etiology and pathological mechanism
  • Disease classification research
  • Medical efficacy evaluation
  • Drug toxicology evaluation

Advantages of Us

  • One-stop FAs analysis solution
  • Short experimental period
  • Detailed analysis repor

CD BioGlyco has been focusing on research in the field of SCFAs. Our FAs analysis services have been widely recognized by researchers, and we will continue to explore the needs of different customers. If you are interested in our SCFAs analysis services, please feel free to contact us.


  1. Dalile, B.; et al. The role of short-chain fatty acids in microbiota-gut-brain communication. Nature Reviews Gastroenterology & Hepatology. 2019, 16(8): 461-478.
  2. Ribeiro, W.R.; et al. Use of gas chromatography to quantify short chain fatty acids in the serum. Colonic Luminal Content and Feces of Mice. 2018, 8(22).
This service is for Research Use Only, not intended for any clinical use.

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