Sorbose, a monosaccharide ketose, is usually in the form of a pyran-type ring. Sorbose is mainly synthesized by microbial fermentation method and has an extremely important physiological role and application value, widely used in pharmaceutical, cosmetic, food, and other industries. Further study of the structure-function relationship of sugar chains will accelerate the development of glycobiology. As a new target for drug development for hypertension, hyperglycemia, hyperlipidemia, and other diseases, a comprehensive analysis of sorbose is necessary for a better in-depth study of its conformational relationship.
Fig.1 α-L-sorbose, and their three conformer classes generated by the rotation of the exocyclic CH2OH group. (Palivec, et al., 2020)
Since sorbose and sorbitol are difficult to separate, rigorous separation and characterization techniques need to be developed. We use a variety of large-scale instruments to provide you with the most convenient and fastest analysis service.
Fig.2 The flowchart of psicose analysis. (CD BioGlyco)
Paper Title: Simultaneous quantitative analysis of sorbitol and sorbose in the fermentation broth with Fourier transform infrared spectroscopy
Journal: Applied Spectroscopy
Results: This paper demonstrates a rapid and noninvasive method to simultaneously monitor sorbitol and sorbose concentrations in broth. The infrared spectra of sorbitol and sorbose in the aqueous solutions are shown in Fig.3. The relevant absorbance bands are situated at 1416, 1081, and 1047 cm-1 for sorbitol and 1160, 1108, and 1052 cm-1 for sorbose, respectively. There is a prominent sorbitol peak at 1081 cm-1 and a sorbose peak at 1052 cm-1, which are relatively isolated to be ideal candidates for quantitative analysis. Attenuated full repeat FTIR is an effective technique for both qualitative and quantitative monitoring of biological processes.
Fig.3 Infrared spectra of sorbitol and sorbose. (Qiu, et al., 2001)
CD BioGlyco attaches great importance to the analysis of Carbohydrate Metabolism. Moreover, we provide efficient Disaccharide Analysis Services, Oligosaccharide Analysis Services, and Polysaccharide Analysis Services. please feel free to contact us.