Hyaluronidase Injection Analysis Service

Hyaluronidase Injection Analysis Service

Ensuring Quality Through Professional Hyaluronidase Injection Analysis

Hyaluronic acid (HA) is a polysaccharide found in the body in connective tissues, joint fluids, and eyeballs. Hyaluronidase is an enzyme that serves to degrade HA in living organisms. Hyaluronidase can break down HA into smaller molecules, thereby regulating the concentration and molecular weight of HA and affecting the structure and function of the extracellular matrix. Hyaluronidase is crucial for the breakdown of the extracellular matrix and the facilitation of tissue healing. CD BioGlyco offers a specialized hyaluronidase injection analysis service, leveraging our extensive expertise and years of experience in Pharmaceutical Analysis. By analyzing the parameters of composition, activity, and stability of hyaluronidase injections, it is possible to determine their release behavior, drug metabolism, and pharmacodynamic properties in vivo.

  • We assess the quality of hyaluronidase injection by measuring its enzyme activity. The methods we commonly use include a colorimetric method, fluorescence method, radioactivity assay, and so on.
  • The protein content of hyaluronidase injection is quantified using colorimetric, BCA, and Lowry methods. The bicinchoninic acid assay (BCA) and Lowry protein assay determine the protein content by colorimetrically measuring the absorbance of the purple compound produced by the complexation reaction of the protein with copper ions.
  • We perform molecular weight determination of the enzyme in hyaluronidase injection by gel electrophoresis or mass spectrometry.
  • We offer hyaluronidase quantification services using colorimetric methods, enzyme-linked immunosorbent assay (ELISA), and high-performance liquid chromatography (HPLC).
  • The impurities and purity in hyaluronidase injection are analyzed by gel electrophoresis, HPLC, and mass spectrometry.

Fig.1 Hyaluronidase injection analysis service. (CD BioGlyco)Fig.1 Hyaluronidase injection analysis service. (CD BioGlyco)

Publication Data

Technology: Fluorescence spectroscopy

Journal: Therapeutic Enzymes: Function and Clinical Implications.

Published: 2019

Results: This article discusses the functions and clinical applications of therapeutic enzymes. The section on hyaluronidase describes the distribution and application of hyaluronidase. Hyaluronidase is an enzyme that degrades HA, an essential component of the extracellular matrix. Hyaluronidase is widely found in nature, including bacteria, insects, snakes, fish, and mammals. In humans, six different hyaluronidases have been identified. Hyaluronidase is used clinically as an adjuvant to increase the bioavailability of drugs, to treat exudates, or for therapeutic purposes such as fillers. The role of hyaluronidase in therapy is to degrade HA, thereby improving the symptoms of the disease in question.

Fig.2 Structure of HA and cleavage by hyaluronidase. (Weber, et al., 2019)Fig.2 Structure of HA and cleavage by hyaluronidase. (Weber, et al., 2019)


  • Hyaluronidase injection is often used as an adjunct to improve the bioavailability of other drugs.
  • Hyaluronidase degrades HA, which can be applied to facilitate the diffusion and absorption of the drug in the tissue interstitium and reduce the accumulation of the drug at the injection site.
  • Hyaluronidase injection can be used as a cosmetic filler to fill in wrinkles and sunken areas of the face to improve the appearance and texture of the skin.


  • We have extensive experience working with diverse samples, which ensures the robustness of our analytical methods and guarantees accurate and reproducible results.
  • Our team of specialists possesses a wealth of expertise in efficiently analyzing samples within tight timeframes, showcasing our proficiency in this area.
  • Our analytical methods are highly specific and can distinguish hyaluronidase from other related substances to avoid misclassification and interference.

CD BioGlyco is a leading biotechnology company that has been at the forefront of providing hyaluronidase injection analysis services. We boast a dedicated team of exceptional scientists and researchers who are unwavering in their commitment to advancing drug research. If you are interested in our service, please contact us for more details.


  1. Weber, G.C.; et al. Clinical applications of hyaluronidase. Therapeutic Enzymes: Function and Clinical Implications. 2019, 255-277.
This service is for Research Use Only, not intended for any clinical use.

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