The rapid update of RNA-seq technology has accelerated transcriptome analyses in new times with higher efficiency and lower cost. Unlike the first-generation sequencing methods based on Sanger sequencing, which used fluorescently labeled or radioactive dNTPs, the researchers used a recently discovered luminescent method named pyrosequencing for DNA sequencing. It laid the foundation for the emergence of NGS. NGS uses "sequencing by synthesis" technology, making it possible to sequence hundreds of thousands of nucleic acid molecules at once and analyze the transcriptome and genome of a species in detail. Therefore, it is also called high-throughput sequencing, which can easily detect unknown gene expression sequences, with many advantages such as fast speed, low cost, and high precision.
Fig.1 Milestone events in the field of RNA sequencing. (Hong, et al., 2020)
CD BioGlyco has invested a lot of effort in RNA sequencing research and has a wealth of experience. The simple NGS workflow we provide is shown as follows:
Fig.2 Workflow of simple NGS. (CD BioGlyco)
CD BioGlyco offers different NGS-based glycogene expression profiling services. There are many opportunities to use NGS in your research:
The technology directly sequences the whole DNA material in a sample, which is essential for identifying the relationship between gene function and disease. CD BioGlyco provides a complete whole-genome sequencing service to assist you in a more in-depth study of glycogenes.
RNA-seq is an essential tool for analyzing differential glycogene expression and studying differential splicing of mRNA at the whole transcriptome level. You fully trust the RNA-seq service provided by us will make your research in glycogenomics much easier.
Chromatin immunoprecipitation (CHIP) is often used in the study of transcription factor binding sites and histone modification sites. CD BiGlyco provides specialized CHIP sequencing technology to help you efficiently study protein-DNA interactions on a genome-wide scale.
It is mission-critical for CD BioGlyco to provide flexible, innovative, and scalable solutions to clients. As an international company that places a high value on collaborative interaction, fast delivery of solutions, and high-quality service, we are constantly improving. We have a comprehensive Glygenomics Platform to solve the challenges in glycobiology for our clients. If you are interested in our services, please feel free to contact us for more comprehensive information.