Modern pharmacological studies have shown that Polygonatum polysaccharide has a variety of physiological activities. It can scavenge free radicals that are harmful to the body. It can protect cells from damage by reducing oxidative stress by activating the SIRT1/AMPK/PGC-1α signaling pathway. In vitro and in vivo studies have demonstrated that Polygonatum polysaccharide may enhance immunity by regulating the TLR4-MAPK/NF-κB signaling pathway, thus exerting anti-tumor effects. In addition, Polygonatum polysaccharide can play an anti-inflammatory role through the TLR4/MyD88/NF-κB signaling pathway and the AMPK signaling pathway. It also has a certain intervention effect on osteoblast growth and differentiation. It plays an important role in promoting osteoblast differentiation and bone formation. In addition to this, it is also active in improving learning ability, memory, hypoglycemia, kidney protection, and bacteriostasis.
CD BioGlyco is committed to creating high-quality assays. The analysis of the polysaccharide structure is of great significance to the study of its conformational relationship as well as product development. We have a well-established testing instrumentation and a professional biochemistry research team. Our assays include sugar residue sequences, monosaccharide composition, molecular weight, glycosidic bonding, etc.
Fig.1 Polygonatum polysaccharide testing program. (CD BioGlyco)
We have a variety of standards including glucose, fructose, fucose, fucose, rhamnose, arabinose, and others. We use HPLC, GC-MS, and HPAEC-PAD to analyze monosaccharide composition. Our monosaccharide composition analysis includes almost all monosaccharides, most oligosaccharides, and more.
We use HPGPC to detect the molecular weight distribution. This method employs HPGPC coupled with multiple tandem gel columns with a refractive index detector (RID). It calibrates the different peak times of the effluent components and compares them with the standard curves prepared from 10 standards to obtain Mw, Mp, and Mn data at the same time. The polymerization profiles are calculated. We also detect the molecular weight by other methods.
We mainly use FT-IR, HPLC, NMR, HPAEC-PAD, and GC-MS to analyze polysaccharide structures. Our NMR database has covered most glycosidic bond structures. Monosaccharide conformations and functional groups are also detected.
Paper Title: Physicochemical, morpho-structural, and biological characterization of polysaccharides from three Polygonatum spp
Technology: HPAEC, HPGPC, NMR
Journal: RSC Advances
Results: The functional groups of the three Polygonatum polysaccharides were characterized by infrared spectroscopy. The results showed that the spectra were similar. The classical polysaccharide absorption peaks were in the range of 500-4000 cm-1. The strong peak at near 3500 cm-1 revealed the O-H stretching vibration. The molecular weights were measured by HPGPC and RID. The results showed that all three polysaccharides presented one major symmetric elution peak with respective Mw values of 8.5×103 Da, 8.7×103 Da, and 1.0×104 Da.
Fig.2 HPGPC chromatograms (A), FT-IR spectral (B), and triple-helical conformation analysis (C) of three polysaccharides. (Bai, et al., 2021)
CD BioGlyco is committed to providing support and assistance to our partners in Carbohydrate Metabolism analysis. In addition to Polygonatum polysaccharide, we also have Sea Cucumber Polysaccharide and Glycogen Analysis Services. Welcome to contact us and talk with us! We are happy to answer any questions you may have about polysaccharide analysis.