Glycan Sequencing

Glycan sequencing is an indispensable part of glycobiology research. CD BioGlyco has a rich variety of glycobiology-related enzymes and systematic identification methods to provide customers with efficient and accurate research services. we have confidence to be your essential research assistant in the field of glycobiology.

Background

As one of the most common protein post-translational modifications (PTMs) in mammals, the alteration of protein glycosylation has been observed in multiple human diseases and may potentially be candidate markers of disease progression. Compared with the development of proteomics, the progress in glycomics is relatively slow, the reason being that the structure and form of glycans are very complicated. There are many isomeric glycans in biological samples at the same time, which adds another challenge to structural glycomics, requiring analytical tools that can provide structural and sequence details from various glycans. In order to fully understand the biological properties of specific glycans in glycoproteins or other glycoconjugates, it is necessary to understand its primary sequence and structure. At present, commonly used analytical methods include capillary electrophoresis (CE), mass spectrometry (MS) and high- performance liquid chromatography (HPLC). These methods are frequently combined with exoglycosidase digestion technology.

Enzymatic sequencing procedure for the identification of Lewis structures, demonstrated for an α1-acid glycoprotein-linked N-glycanFig 1. Enzymatic sequencing procedure for the identification of Lewis structures, demonstrated for an α1-acid glycoprotein-linked N-glycan (Christoph, K.; et al. 2019)

Services

CD BioGlyco provides numerous highly specific exoglycosidases which are usually used to determine the sequence and structure of glycans. This enzymatic analysis is a powerful technique and applied either sequentially or in a matrix array. Exoglycosidase specifically removes terminal glycosyl groups from the non-reducing ends of glycans without cutting the internal bonds between monosaccharides. The bonds and sugar groups of the removed glycan residues can be identified by using various bond-specific exoglycosidases

Applications

  • Disease pathology and progression research
  • Proteins and other biomacromolecules structure & function research
  • Protein heterogeneity research

Advantages of Us

  • A large number of glycobiological enzymes
  • Multiple computer analysis platforms
  • Professional researchers
  • Cost-effective

CD BioGlyco provides a large number of tool enzymes and analysis equipment involved in glycobiology research to meet customers' various experimental requirements. We will always adhere to the principle of customer-centricity, keep full transparency in the experimental process and speed up the research of glycobiology for customers.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

Reference:

  1. Christoph, K.; et al. Enzymatic Sequence Analysis of N-Glycans by Exoglycosidase Cleavage and Mass Spectrometry: Detection of Lewis X Structures. Methods Mol. Biol. 2019.
This service is for Research Use Only, not intended for any clinical use.

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About Us

CD BioGlyco is a world-class biotechnology company with offices in many countries. Our products and services provide a viable option to what is otherwise available.

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