Heparin Lock Flush Solution Analysis Service

Heparin Lock Flush Solution Analysis Service

Unlocking Satisfaction in Heparin Lock Flush Solutions with Precise Analysis

Heparin is a natural anticoagulant that is commonly used to prevent diseases such as thrombosis. Heparin lock flush solution is an injectable solution that is commonly used to maintain the patency of intraventricular lines and prevent thrombosis. It consists mainly of heparin and saline, which has an anticoagulant effect that inhibits blood clotting, thus maintaining the patency of the intraventricular line. CD BioGlyco has been dedicated to delivering exceptional Pharmaceutical Analysis Services to our esteemed clients for an extensive period, and our services have garnered high acclaim from our valued clientele. Currently, there are several methods available for the analysis of heparin lock flushes. These include physicochemical methods, chromatography methods, spectroscopic methods, and biological assays. We utilize these technologies to provide you with superior heparin lock flush solution analysis services.

  • We provide a comprehensive quality evaluation service by assessing parameters such as solubility, specific gravity, viscosity, and more. This analysis enables you to determine crucial properties including density, compatibility with solvents, and stability of the heparin lock flushing solution.
  • We quantify the active ingredients in your samples by high-performance liquid chromatography (HPLC) or gas chromatography (GC) techniques. These chromatography techniques are capable of rapidly and accurately determining the amount and composition of the various active ingredients in heparin lock rinses.
  • In addition to the aforementioned techniques, we employ ultraviolet (UV)-visible (Vis) spectroscopy to further investigate and quantify the target components. This analytical method allowed us to determine the concentration of these components accurately and confirm the presence and identity of these target compounds.

Fig.1 Analytical approach to heparin lock flush solution. (CD BioGlyco)Fig.1 Analytical approach to heparin lock flush solution. (CD BioGlyco)

Publication Data

Technology: Anti-Xa assay and GC

Journal: Journal of Pediatric Surgery

IF: 2.4

Published: 2015

Results: This article examines the chemical stability of heparin, isopropanol, and ethanol line lock solutions. The article explores the chemical and physical stability of these solutions in central venous catheters over 10 days at 2-8°C and 25°C. Samples were prepared over 10 days and stored in a refrigerator at 2-8°C or in a 60% relative humidity cabinet at 25°C. Before any analysis, sample syringes were subjected to a standard mixing process in which 1 mL of air was withdrawn, a combi-stopper was attached, and the syringe was inverted 10 times. The researchers analyzed the samples for visible particle counting using the CLIMET particle counting system to assess the number of particles in solution and analyzed the concentration of heparin in the samples using the anti-Xa assay. The results of the study showed that the addition of isopropanol to heparin and ethanol prevented the formation of precipitates. However, the activity of heparin gradually decreased over time, making this solution unsuitable for prolonged storage.

Fig.2 The applications of heparin. (Zang, et al., 2022)Fig.2 The applications of heparin. (Zang, et al., 2022)


  • Our advanced analytical technology offers exceptional sensitivity in detecting even minute quantities of heparin components within the heparin lock flush solution, guaranteeing precise and dependable outcomes.
  • Our analytical technology has good selectivity and can accurately differentiate between the various components in a heparin lock flush solution, avoiding the influence of interfering substances on the analytical results.
  • Our techniques have been extensively validated and successfully implemented over the past years, demonstrating exceptional reliability and stability.

CD BioGlyco remains committed not only to providing efficient heparin lock flush solution analysis service but also to ensuring timely project completion. We maintain streamlined processes that minimize turnaround times without compromising quality. Please feel free to contact us for more details.


  1. Cullis, P.S.; et al. Chemical stability of heparin, isopropanol, and ethanol line lock solutions. Journal of Pediatric Surgery. 2015, 50(2): 315-319.
  2. Zang, L.; et al. Not just anticoagulation-new and old applications of heparin. Molecules. 2022, 27(20): 6968.
This service is for Research Use Only, not intended for any clinical use.

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