Background

Glycosylation is one of the most common protein post-translational modifications. At least 50% of human proteins are glycosylated. N-, O-linked glycans are required to maintain the biological functions of proteins. Insufficient glycosylation can lead to animal and plant diseases. Therefore, characterizing the degree of glycosylation on proteins is an important step in understanding the diagnosis and treatment of diseases. N-, O-glycosylation is also closely related to various biological events, including cancer metastasis, virus infection and antibody-antigen interactions, so the accurate determination of site occupancy is essential to fully understand the impact of protein glycosylation on human health. Unlike N-glycosylation, it is more difficult to analyze O-linked glycans due to the lack of universal enzymes for releasing O-glycans from proteins and the high degree of heterogeneity in their occupation.

Glycoprotein analysis requires the determination of glycosylation sites and the glycan structure associated with each site. In recent years, a wide range of analysis methods based on mass spectrometry have been developed. These methods require high resolution and sensitivity so that glycosylation sites and micro-heterogeneity information can be obtained.

Integrative Multidimensional Histology Solutions for Resolving Site Occupation

Through high-precision mass spectrometry (MS) combined with bioinformatics analysis, we provide quantitative assessment services of the actual occupancy of glycosylation sites in target proteins, helping clients to reveal the degree of modification of each potential glycosylation site.

High-Throughput Glycosylation Site Identification

Kinetic Studies of Site-Specific Modifications

• Our researchers provide maps of dynamic changes in glycosylation site occupancy through time-series sampling analysis. Moreover, we combine bioinformatics tools to help our clients predict key regulatory nodes of the glycan chain synthesis pathway.
• By comparing the difference in peptide mass before and after deglycosylation, we provide a quantitative analysis of the occupancy of glycosylation sites.
• For in-depth analysis of specific loci, we use advanced analytical tools to provide high-resolution glycosylation data.

Workflow

Our service process includes sample pre-treatment (e.g., enzymatic digestion, glycan release, and enrichment), MS data acquisition, glycosylation site identification, site occupancy calculation (i.e., the ratio of modified sites to total sites), and further analysis of glyco-heterogeneity, glycan structure, and dynamic changes.

Applications

• Our service can be used to optimize the glycosylation of the Fc segment of antibody drugs to enhance the antibody-dependent cell-mediated cytotoxicity (ADCC) effect.
• Our services can be used for vaccine antigen design and screening of optimal glycosylation modification epitopes.
• Our services can be used for biosimilar glycosylation fingerprinting and glycosylation stability monitoring.

Advantages of Us

• We are a well-experienced glycobiology company that supports a wide range of MS and analytical software to meet different research needs.
• We provide not only raw data but also data processing reports such as visual data analysis, interoperability network analysis, and so on.
• We offer customized solutions based on client needs, ensuring the accuracy and reliability of glycosylation site analysis results.
• Our professional team has rich experience in glycosylation research and can provide clients with comprehensive technical support and consultation services.

Publication Data

Frequently Asked Questions

CD BioGlyco is an experienced company in the analysis of glycosylation site occupation. Our multi-functional analysis platform and powerful database can provide customers with a diverse portfolio of glycosylation analysis services, and our excellent services have helped us gain a good reputation worldwide. We hope to start the exploration of glycobiology with you.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

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