The occurrence and development of many diseases such as cancer are often accompanied by abnormal changes in the glycosylation sites and carbohydrate chains of proteins, so characterizing glycosylation becomes extremely necessary in protein research. CD BioGlyco has advanced technology platforms and a complete database to provide customers comprehensive and time-saving services in glycosylation analysis. We have confidence to be your essential research assistant in the field of glycobiology.

Background

In recent years, the role of O-glycosylation in different proteins or other biological macromolecules has been intensively studied. In order to further understand how O-glycosylation modification confers receptors function, the research on macroheterogeneity (glycosylation site occupancy) becomes an essential step. It is critical to first determine whether the predicted O-glycosylation sites are modified and the relative amounts of the various glycoforms of O-glycosylation at each of those sites. Glycosylation sites analysis can not only obtain information intuitively about the glycosylation changes of numerous disease markers, but also make the further carbohydrate chain structural analysis more direct and convenient.

Unlike N-glycosylation, analyzing O-linked glycans is traditionally considered a difficult task for many reasons, including i) Lack of consensus sequence for the glycosylation sites on the polypeptide. ii) High heterogeneity both in the number of glycans and the extent of their occupancy. iii) Lack of universal enzyme to release O-glycans from the protein. High resolution and sensitivity of mass spectrometry are required for the discovery and confirmation of glycosylation sites.

Services

In general, O-linked glycoproteins are analyzed in three different ways, and these strategies are mainly carried out using a variety of analytical platforms either stand alone or in combination with other techniques.

• Glycan profiling: release of O-glycans by enzymatic or chemical treatment, followed by subsequent characterization.
• Bottom-up approach: digestion of glycoproteins into peptides followed by analysis of the glycan attached peptides.
• Top-down approach: analysis and characterization of glycans on intact glycoproteins.

O-glycosylation site occupation analysis in CD BioGlyco including but is not limited to the following MS based glycomics technologies.

• Tandem mass spectrometry (MS²)
• Higher-energy collisional dissociation (HCD)
• Electrospray ionization (ESI)
• MALDI in-source decay (ISD)
• Ion mobility-mass spectrometry (IM-MS)
• Collision-induced dissociation (CID)
• Traveling wave ion mobility-mass spectrometry (TWIMS)
• Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)
• Electron capture dissociation (ECD) and electron transfer dissociation (ETD)

Workflow

Applications

• Our service can be used to analyze abnormal glycosylation sites in cancer and neurodegenerative diseases to reveal the mechanism of disease development.
• Our services can be used to explore the role of protein glycosylation in cell signaling, immune response.
• Our services can be used to assess the effect of antibody drugs or enzyme inhibitors on glycosylation modifications and to optimize drug target screening.

Advantages of Us

• High sensitivity: Our company uses high-resolution MS to realize high-sensitivity detection and complete projects with high quality.
• High throughput: Our automated equipment allows clients to test and analyze multiple glycoprotein samples at the same time, greatly shortening project cycle time.
• Our company uses multiple ion fragmentation modes combined with a glycan-specific database to enhance the detection rate of low-abundance sites.

Publication Data

Frequently Asked Questions

CD BioGlyco provides a variety of comprehensive glycosylation research technology platforms to meet customers research needs. For a scientific research project, we guarantee one-stop service and maintain transparency in the whole process.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

Associated Services