Analysis of Glycolytic Metabolic Flux in Cancer Cells

Analysis of Glycolytic Metabolic Flux in Cancer Cells

The metabolism of cancer cells is regulated by dynamic and complex networks. Metabolomics can only analyze static metabolites, while metabolic flux analysis techniques can describe the metabolic state of cancer cells at a dynamic level. Glycolysis is the process of decomposing one molecule of glucose into two molecules of pyruvate, and the flux analysis of glycolysis is to analyze the rate of this process and determine the direction of glucose metabolism in the process. CD BioGlyco uses isotope-labeled tracers for glycolytic metabolic flux analysis to grasp the metabolic status of the glycolytic pathway throughout cancer progression.

What We Do

Metabolic flux analysis is the main research method of metabolic fluxomics, among which the metabolic flux analysis of glycolysis is more in the primary position in the study of glucose metabolism in cancer cells. We provide metabolic flux analysis of glycolytic pathways based on the MOPCGM platform.

We provide steady-state glycolytic metabolic flux analysis and non-steady-state glycolytic flux analysis. Among them, steady-state glycolytic flux analysis has an independent marker collection mode, which takes a long time. Non-steady-state glycolytic flux analysis takes less time but requires more time points to be analyzed. You can choose according to your needs.

For glycolytic flux analysis, the labeling methods we can provide are as follows:

[U-13C]-Glucose [U-13C]-Fructose
[1-13C]-Glucose Parallel labeling

The glycolytic metabolites we are able to label are as follows, including but not limited to:

Glucose 6-Phosphate Glucose 1-Phosphate
Fructose 6-Phosphate Fructose 1,6-Bisphosphate
3-Phosphoglyceric Acid 2-Phosphoglyceric Acid
Phosphoenolpyruvic Acid Pyridoxal 5-Phosphate

The workflow of metabolic flux analysis

Analysis of Glycolytic Metabolic Flux in Cancer Cells


  • Glycolytic metabolic flux analysis report
  • Glycolytic metabolic flux differential analysis report
  • Glycolytic Metabolic flux hierarchical cluster analysis report
  • Glycolysis Bi-plot analysis report

Our Advantages

  • We provide complete data and detailed analysis results including raw data, metabolic flux analysis data, metabolic flux difference analysis data, differential compound hierarchical cluster analysis data, and Bi-plot analysis data.
  • We guarantee raw data and analysis reports within one month of your sample arrival.
  • We are able to monitor the glycolytic pathway in real-time.


  • Analysis of glycolytic changes in cancer cells before and after gene therapy.
  • Evaluation of glycolytic metabolism regulator function.
  • Application of glycolytic metabolism engineering in cancer therapy.

CD BioGlyco can provide glycolytic metabolic flux analysis of cancer cells from various sample sources, helping customers understand the metabolic status of glycolytic pathways in real-time. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.

This service is for Research Use Only, not intended for any clinical use.

About Us

CD BioGlyco is a world-class biotechnology company with offices in many countries. Our products and services provide a viable option to what is otherwise available.

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