Nanotechnologies for the Detection of Glycopeptides
Overview
Many low concentrations of glycoproteins/glycopeptides in serum or tissue extracts are biomarkers with high clinical sensitivity and specificity, and these biomolecules could provide important information for disease pathogenesis exploration, disease marker discovery, and protein-based drug development. However, it is still a challenge for glycoproteomics to selective isolation and enrichment of glycoproteins/glycopeptides from complex biological samples due to their low concentrations. Currently, nanotechnologies have been widely used for the detection of glycopeptides due to their high sensitivity and specificity.
Nanomaterials possess larger surface areas than bulk materials because of their smaller size. In the context of rapid and sensitive detection, this feature provides additional activation sites for glycopeptide enrichment and unique optical properties. The most promising property of nanomaterials is the degree to which their shape, size, and morphology could be precisely controlled to enhance the electronic, optical, or magnetic capabilities typically required in certain applications. Several nanoplatforms are currently available at CD BioGlyco for the separation of glycopeptides in complex samples with higher sensitivity and specificity.
Fig.1 Overview of different nanomaterials (outside the blue circle) used in combination with biomaterials/biomolecules (inside the blue circle) for various applications (in yellow circle). (Dosekova, 2017)
Exploring the Story of Nanomaterials and Glycopeptide Enrichment
Our distinguished expertise at CD BioGlyco in nanotechnologies for glycopeptide detection is anchored in several cutting-edge materials, each meticulously optimized to address specific requirements for superior glycopeptide enrichment and subsequent analysis:
CD BioGlyco strategically employs precisely engineered nanoparticles, including advanced magnetic nanoparticles and tailored polymeric nanoparticles, to achieve highly selective capture and robust concentration of glycopeptides from even the most intricate biological samples.
We meticulously utilize sophisticated mesoporous materials, distinguished by their highly ordered and precisely controllable porous structures, for advanced glycopeptide enrichment. These materials boast an exceptionally high surface area and precisely engineered pore sizes, enabling sophisticated size-exclusion and highly specific affinity-based enrichment strategies.
CD BioGlyco utilizes graphene's extraordinary adsorption capacity and inherent chemical stability to enrich glycopeptides. The unique two-dimensional atomic structure of graphene provides an abundance of accessible active sites for strong yet specific interactions with glycopeptides, significantly augmenting enrichment efficiency.
CD BioGlyco has developed a strategy to apply CNTs in the detection of glycopeptides to enhance glycan signaling. The method has high sensitivity and specificity, and shows good analytical performance in glycoprotein analysis. CNTs provide robust and adaptable platforms for the precise immobilization of various affinity ligands, facilitating the highly selective capture of specific glycopeptide subsets.
CD BioGlyco proudly offers a comprehensive and adaptable suite of services leveraging our cutting-edge nanotechnologies for glycopeptide detection.
- Discovery and validation of glycopeptide biomarkers:We provide robust platforms for the identification and rigorous validation of novel glycopeptide markers, pivotal for discerning various disease states, understanding progression, and evaluating therapeutic responses.
- Targeted glycopeptide analysis: Our services include highly precise and accurate quantification of specific glycopeptides of particular interest.
- Comprehensive glycosylation profiling: We offer in-depth, systematic mapping of complex glycosylation patterns, whether on individual proteins or across entire proteomes, providing invaluable insights into their biological roles.
Workflow
Applications
- Our services are instrumental in the identification, characterization, and rigorous validation of novel glycopeptide biomarkers.
- Our services can be used to precisely elucidate the complex role of glycosylation in countless fundamental biological processes, including key areas such as cell signaling pathways, immune system regulation, cellular recognition events, and processes important for protein folding and stability.
- Our services can be used to accurately characterize biopharmaceutical drug glycoforms.
Advantages of Us
- Our cutting-edge nanomaterial-based enrichment strategies dramatically lower detection limits, enabling the routine analysis and confident identification of low-abundance glycopeptides.
- Our approach minimizes background noise and significantly reduces matrix interference, ensuring exceptionally clean samples for downstream analysis.
- Our meticulously optimized protocols, combined with the inherent efficiency of nanomaterial interactions and our advanced automated platforms, facilitate the rapid and concurrent processing of multiple samples.
Publication Data
DOI: 10.1002/med.21420
Journal: Medicinal Research Reviews
Published: 2017
IF: 10.9
Results: This paper details the synthesis of gold nanoparticles (AuNPs) and gold nanorods (AuNRs) and how these particles can be functionalized by glycans such as lactose and N-acetyl-D-glucosamine neoglycoconjugates. Glycan-coated nanoparticles selectively bind to carbohydrate-binding proteins (lectins), even in complex, protein-rich biological media. This is crucial for the 'selectivity' of glycopeptide enrichment. It emphasizes that specific glycosyl-nanomaterial interactions can be used to isolate desired glycopeptides from challenging biological matrices.
Fig.2 Different types of nanomaterials. (Dosekova, et al., 2017)
Frequently Asked Questions
With a high level of research capability and professionally educated researchers, CD BioGlyco has developed a variety of nanotechnologies and successfully applied them to the detection of low-abundance endogenous glycoproteins/glycopeptides. If you have a demand in this field, please contact us for technical support and we will be delighted to provide you with the best service.
Associated Services
To comprehensively support your research and development aspirations within the vast field of glycoproteomics and beyond, CD BioGlyco offers a range of highly complementary services related to our nanotechnologies for glycopeptide detection.
Reference
- Dosekova, E.; et al. Nanotechnology in glycomics: applications in diagnostics, therapy, imaging, and separation processes. Medicinal Research Reviews. 2017, 37(3): 514-626. (Open Access)
