CD BioGlyco is committed to improving the sensitivity in the detection of complex glycans structures by developing various approaches, including glycan derivatization. We provide a highly skilled specialist service using advanced mass spectrometry (MS) techniques and a variety of efficient derivation strategies to enable research teams to perform analysis for a wide range of samples.

Introduction

Just like proteins observed in nature, additional structural diversity can be imparted to glycans by modifying the hydroxyl group of glycans with phosphate, sulfate, or acetyl esters, which is able to improve the efficiency of separation and detection to obtain accurate and reliable structural information of various glycans. A general method for detecting the glycans on proteins includes periodate oxidation of ortho hydroxyl groups followed by the formation of Schiff bases with amine or hydrazide-based probes. This chemical modification procedure can be used to characterize glycoproteins in gels. Over the past years, various gas-liquid chromatography (GLC) strategies were developed for glycomic quantitation. The coupling of GLC and MS is the most efficient method to obtain information on linkage and composition. Most importantly, the efficiency of these methods was greatly improved when monosaccharides were first chemically modified on their hydroxyl and aldehyde groups. Reducing the aldehyde of a free monosaccharide and then acetylating its hydroxyl group provides a derivative called "peracetylated alditol acetate". These modified monosaccharides can be easily analyzed by GLC and MS.

Derivation Strategies

CD BioGlyco has developed various hydroxyl group derivatization methods for the analysis of glycans by mass spectrometry and other detection techniques. The strategies we provide include but are not limited to:

• Methylation of hydroxyl groups. The capping of hydroxyl groups to produce methyl ethers is a common modification, which can be used for the structural analysis of glycans.
• Esterification of hydroxyl groups. Esterification is an important element of glycan structure variation. The most important types of sugar esters are phosphate esters, acyl esters, and sulfate esters.
• Deoxygenation of hydroxyl groups. Hydrogen atoms can replace hydroxyl groups of monosaccharides to form deoxy sugars.

Applications

• Research on analysis of glycans structures
• Applications in improving detection efficiency
• Research on separation of complex glycans

Advantages of Us

• State-of-the-art instrumentation
• Highly trained personnel and cutting edge technology
• Extensive experience in glycan derivation service
• Detailed after-sales service

Staffed with professional bioanalysts and technicians, CD BioGlyco provides reliable and efficient technical support to your project. We serve all investigators from academic institutions and biotechnology companies. If you need our scientific support, please contact us for further information.

Reference:

1. Chang, S.J.; et al. An efficient approach to derive hydroxyl groups on the surface of barium titanate nanoparticles to improve its chemical modification ability. Journal of Colloid and Interface Science. 2009, 329(2): 300-305.