CD BioGlyco continuously improves the ability to comprehensively characterize highly complex glycan mixtures, aiming to provide customers with high-quality services in glycan separation and purification. We look forward to working with you and providing you with comprehensive scientific services in the field of glycobiology.
The complexity of glycosylation patterns is attributed to the multiple glycosylation sites of proteins and their related microheterogeneity. This structural variation and high complexity ultimately determine the function and activity of glycoproteins. The abnormal glycosylation of glycoconjugates is related to many diseases, such as immune deficiencies, hereditary disorders, and cardiovascular diseases, Therefore, the development of analytical tools that can effectively analyze glycans is helpful for the diagnosis of these diseases and the understanding of these diseases at the molecular level.
At present, the characterization of glycans is usually attained by MS. Although MS and MS/MS technologies can provide a wealth of structural information, they can not readily identify the isomeric glycan structure in the absence of high-order MS/MS (MSn). Several separation methods, including hydrophilic interaction chromatography, high pH anion exchange chromatography, and GC have been used to analyze the structure of isomeric glycans. However, CE has high separation efficiency and resolutions and is able to separate closely related glycan structures by charge-to-size ratio. CE technology has attracted extensive attention in recent years and plays a unique role in glycoscience research.
Fig.1 Basic diagram of the capillary electrophoresis system commonly used to separate APTS-labeled glycans. (Lu, 2018)
Capillary electrophoresis has emerged as a powerful method for glycan analyses. Currently, several methods have been developed by CD BioGlyco based on CE technology to determine the structure and linkage composition of unknown glycans in samples. These techniques can be divided into the following categories:
CD BioGlyco persists in the innovation in instruments and instrument applications, provides customers with a variety of glycan separation and purification solutions, and continuously improves the solutions to meet the specific needs of customers. Please contact us directly for more details if you need scientific assistance.