Rhamnan Analysis Service

Rhamnan Analysis Service

Biological Activities of Rhamnan

Rhamnan is a polysaccharide that is polymerized from monosaccharides such as rhamnose. Since pure rhamnose crystallizes in two forms, α-type and β-type, the backbone structure of rhamnan also shows these two forms. Rhamnan has a variety of biological activities, including antioxidant, antibacterial, antiviral, antitumor, hypolipidemic, and immune regulation. Studies have shown that rhamnan can promote cell regeneration and repair, and has a protective effect on many organs, including the digestive tract, cardiovascular system, and nervous system. In addition, it can also be used as an additive in cosmetics and food. Therefore, rhamnan has a broad application prospect in medical and biological fields.

Rhamnan Analysis Service at CD BioGlyco

Rhamnan has great development potential and CD BioGlyco has extensive experience in the Analysis of Polysaccharides. Therefore, we provide comprehensive rhamnan analysis services.

  • Molecular weight determination
    CD BioGlyco provides high-performance gel permeation chromatography (HPGPC) to determine the molecular weight and molecular weight distribution of purified rhamnan and provide timely data reports to clients.
  • Structural analysis
    We provide ultraviolet-visible spectrum (UV), infrared spectroscopy (IR), and nuclear magnetic resonance (NMR) analysis services to every client. Among them, the UV spectrum is to analyze the purity of rhamnan. Infrared and multi-dimensional NMR technology can increase the ability to determine the backbone structure of the polysaccharide chain and the conformation.
  • Microscopic surface structure
    For the surface condition of polysaccharide particles, we provide scanning electron microscopy (SEM) technology.
  • Thermal stability analysis
    We have a professional thermogravimetric (TG) analyzer and differential scanning calorimeter (DSC) to analyze the thermal stability of polysaccharides.

Fig.1 Analysis procedures of rhamnan. (CD BioGlyco)Fig.1 Analysis procedures of rhamnan. (CD BioGlyco)


Paper Title: Purification, characterization, and biological activities of exopolysaccharides from Lactobacillus rhamnosus ZFM231 isolated from milk

Technology: UV, IR and NMR analysis

Journal: LWT-Food Science and Technology

Published: 2021

Results: Ultraviolet-visible spectrum (UV-Vis) spectra of exopolysaccharides (EPS) solutions were recorded on a UV spectrophotometer scanning from 200 to 800 nm, aiming to estimate the purity of the purified components. Infrared spectroscopy (IR) spectra of the purified EPS were recorded on a Nicolet 380 infrared spectrometer at the wave number range of 4000-400 cm−1 using a KBr pellet method. 1H NMR spectra of EPS were recorded on a Bruker Avance 600 spectrometer, and 13C NMR spectra of EPS were recorded on a Bruker Avance 400 spectrometer.

Almost all of the IR spectra, 1H NMR, and 13C NMR spectra can be used for the analysis of the glycosidic bond configuration of polysaccharides (Fig.2). From the plot, it is clear that α-glycosidic linkage is the main linkage between the monosaccharide units in EPS has both α- and β-glycosidic linkages.

Fig.2 FT-IR spectra(a) and 1H NMR spectra(b) of EPS. (Hu, et al., 2021)Fig.2 FT-IR spectra (a) and 1H NMR spectra (b) of EPS. (Hu, et al., 2021)


  • In drug discovery and development, Rhamnan analysis services are used in the study of antiviral drugs.
  • Rhamnan analysis services are used to assist in the reaearch of immunoregulatory processes in the human body.
  • Rhamnan analysis is used in the development of pharmaceutical intermediates.


  • Comprehensive analysis of rhamnan facilitates the development of carbohydrate-based drugs.
  • Our comprehensive analysis services and numerous methods save our clients' time and cost.

CD BioGlyco is a leader in the field of polysaccharide analysis, providing best-in-class polysaccharide analysis services to our clients. We have accumulated years of experience in rhamnan analysis. If you are interested in our services, please feel free to contact us for details.


  1. Hu, S.M.; et al. Purification, characterization, and biological activities of exopolysaccharides from Lactobacillus rhamnosus ZFM231 isolated from milk. LWT-Food Science and Technology. 2021, 147: 111561-111570.
This service is for Research Use Only, not intended for any clinical use.

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