Glycoprotein Quantification

Background

Protein glycosylation is one of the major post-translational modifications that can significantly impact protein folding, distribution, stability, conformation and activity. Glycoprotein abundance, glycosylation degree and glycan structure changes are closely related to many diseases (such as genetic diseases, immunodeficiency, cardiovascular diseases, and cancer). The research on glycoproteomics has become popular, increasing the demand for the development of quantitative glycomics strategies. These strategies need to be able to sensitively monitor the abundance and structural changes of glycans. The role and characteristics of glycans in biological systems can be clarified using these strategies.

In the past, people focused their research on the enrichment and separation of glycoproteins/glycopeptides, identification of glycosylation sites, and glycan structure analysis. So far, the development of quantitative glycomics has lagged slightly behind other studies. At present, a combination of multiple analytical techniques (such as capillary electrophoresis, liquid chromatography and mass spectrometry) is mainly used to analyze glycoprotein quantitatively. To enhance the sensitivity in spectroscopy and mass spectrometry, it is necessary to label with ionic and/or hydrophobic reagents during the analysis. Labeling with stable isotope reagents has also been considered a feasible strategy to achieve relative quantification.

Fig 1. Flow chart for quantitative glycan analysis using isotopic labeling during the permethylation stepFig 1. Flow chart for quantitative glycan analysis using isotopic labeling during the permethylation step (Orlando, R. 2012)

Services

CD BioGlyco provides a variety of technology platforms for quantitative analysis of glycoproteins, mainly including fluorescence spectroscopy, capillary electrophoresis, laser-induced fluorescence detection, hydrophilic interaction chromatography with fluorophores, and mass spectrometry. Our researchers can provide the best experiment design according to our customer's research needs. We provide the following quantitative analyses.

  • Quantitative analysis of glycans derivatized with reductive amination chemistry
  • Quantitative analysis of glycans derivatized with reagents that introduce a cationic charge
  • Quantitative analysis of glycan hydrazones
  • Quantitative analysis of permethylated glycans
  • Quantitative glycomics through stable isotopic labeling
  • Metabolic labeling quantitative glycomics
  • Quantitative glycomics with porous graphitized carbon columns

Applications

  • Glycoprotein structure and function research
  • Biomarker discovery
  • Research on pathogenesis of major diseases
  • Systematic research on glycoproteomics

Advantages of Us

  • High resolution, high reproducibility
  • Continuously updated and upgraded technology platform
  • Rich experience, thousands of successful cases
  • Powerful data analysis tools
  • Short experimental period

CD BioGlyco is a company with rich experience and good reputation in the field of glycoproteomics. Our multi-platform method and well-trained researchers can greatly accelerate customer research and help customers explore glycobiology more comprehensively and accurately.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

References:

  1. Mechref, Y. et al. Quantitative glycomics strategies. Molecular & Cellular Proteomics. 2013, 12(4): 874-884.
  2. Orlando, R. Quantitative analysis of glycoprotein glycans. Methods in Molecular Biology. 2012.
This service is for Research Use Only, not intended for any clinical use.

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