Glycan Analysis: High-Throughput Screening via HPLC

High-throughput screening (HTS) of glycans is an important application of high-performance liquid chromatography (HPLC) for the efficient and rapid characterization of large numbers of glycan samples. By using HPLC, structural modifications of glycans and other related information can be analyzed in a high throughput manner. This enables researchers to efficiently screen and characterize target glycan molecules from a large number of samples. By integrating cutting-edge techniques such as auto sampling systems, the sample handling capacity, data acquisition, and analytical efficiency of HPLC are optimized. The combination of HPLC and HTS provides a powerful platform for accelerated glycan research. This powerful combination enables scientists to gain insight into the nature, structure, and activity of glycans. This deepens our understanding of the properties and biological functions of glycans and also promotes breakthroughs in glycan-related research.

Fig.1 A modern self-contained HPLC. (Wikipedia)

HPLC-Based Glycan HTS Service at CD BioGlyco

We provide HPLC-based glycan HTS services to facilitate rapid and efficient screening of latent biologically active compounds for clients. We also perform structural analysis in conjunction with an autosampler system and other analytical techniques to further understand the characteristics of the glycan samples. The services we offer are as follows:

• High-throughput glycan chain modification analysis
  We combined HPLC with Liquid Chromatography-Mass Spectrometry (LC-MS) to analyze the modifying groups or ligands in glycans. By separating and detecting modified glycan fragments, the type of modification of the glycan can be inferred. By separating and introducing the glycan into the mass spectrometer by HPLC, a mass spectral profile of the glycan is obtained, which makes it possible to determine the information regarding the structure, modifications, and fragmentation patterns of the glycan to be determined.
• High-throughput glycan structure analysis
  We isolated the glycans by HPLC technique and then analyzed the samples collected by fractionation by nuclear magnetic resonance (NMR). Multiple samples can be processed quickly using an automated NMR analysis system. Subsequent data analysis is performed to obtain information on the chemical structure within the glycan molecules, such as linkage patterns, bond lengths, and functional groups.

We analyze the modification and structural information of glycans in a high-throughput manner to help you screen out the target glycan molecules that match your research from a large number of glycans.

Fig.2 Experimental procedure for high throughput analysis of glycans by HPLC coupled with NMR. (CD BioGlyco)

Applications

• Acts as a drug carrier to help improve drug solubility
• Modifying the structure of drug molecules
• Preparation of biomedical materials

Advantages of Us

• Multi-parameter analysis
• High resolution and reliability
• Highly specialized services

CD BioGlyco offers HPLC-based glycan HTS services to meet our clients' needs in glycan research and development. Our HTS services are not only highly automated and efficient but also accurate and reliable. You will get comprehensive and detailed glycan analysis data after the analysis, which will provide strong support and guidance for your research and development work. If you are interested in our services, please contact us for more details.