Neomycin Sulfate Analysis Service

Neomycin Sulfate Analysis Service

Uncover the Essence of Neomycin Sulfate with Precision and Expertise

CD BioGlyco has a long-term focus on research in glycobiology and offers a wide range of Pharmaceutical and Biological Analysis Services to our clients. In particular, neomycin sulfate is used as an aminoglycoside antibiotic, which exerts favorable effects on a wide range of bacteria (e.g., Staphylococcus aureus, Bacillus diphtheriae, and Bacillus anthracis, etc). Therefore, we provide high-quality neomycin sulfate analysis services according to the needs of our clients. The analysis services we provide include but are not limited to the following.

  • Identification of neomycin sulfate
    • Coloring reaction: After dissolving a small amount of the sample to be tested in water, we add hydrochloric acid solution, sodium hydroxide solution, and acetylacetone solution to it in turn. After heating for some time, let it stand, and then add p-dimethylaminobenzaldehyde test solution to it, if the solution shows a cherry red color, it means the sample is neomycin sulfate.
    • Thin-layer chromatography (TLC): The sample to be tested and the neomycin sulfate standard are dissolved separately and expanded on a thin layer plate under the same conditions. Compare the heights of the two spots, if the heights are the same, the sample is neomycin sulfate
  • Content analysis
    The sample to be tested is weighed precisely, and sterilized water is added to make a solution. Then we will use the antibiotic microbiological assay method to determine the content of neomycin sulfate.
  • Neomycin impurity analysis
    Thin-layer chromatography is used to compare the content of neomycin impurities in neomycin sulfate standards and samples.
  • Acidity analysis
    The neomycin sulfate sample is dissolved in water and the pH value is determined using a pH meter. The pH of the neomycin sulfate standard should be 5.0-7.0.
  • Loss on drying
    The samples to be tested are dried under reduced pressure at elevated temperatures to a constant weight. Theoretically, the weight loss of neomycin sulfate should not exceed 6.0%.
  • Neomycin sulfate is usually available in several other states, and we offer their analysis as well.
    • Neomycin sulfate tablets: white tablet form
    • Neomycin sulfate eye drops: clear colorless to slightly yellow liquid

    • They are identified and analyzed in the same way in which neomycin sulfate is. However, the pH of neomycin sulfate eye drops is usually 6.0-7.0.

Fig.1 Neomycin sulfate analysis services. (CD BioGlyco)Fig.1 Neomycin sulfate analysis services. (CD BioGlyco)

Publication Data

Technology: Dispersive spectroscopy (EDS), Rayleigh scattering (RRS) spectroscopy

Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

IF: 4.4

Published: 2017

Results: The authors utilized the glucose-Fehling reagent reaction catalyzed by graphene oxide (GO) to successfully prepare stable Cu2O nanocubic (Cu2ONC) sols. The authors then characterized it using EDS. On this basis, the authors developed a highly sensitive and selective RRS energy transfer (RRS-ET) method for the detection of neomycin sulfate by using Cu2ONC as an RRS spectroscopic probe. The linear range of the method was 1.4-112 μM, and the detection limit was 0.4 μM.

Fig.2 The decreased RRS intensity is linear to neomycin sulfate concentration in the range of 1.4-112 μM. (Ouyang, et al., 2019)Fig.2 The decreased RRS intensity is linear to neomycin sulfate concentration in the range of 1.4-112 μM. (Ouyang, et al., 2019)


  • We provide custom neomycin sulfate analysis solutions according to the client's requirements and we also provide detailed data interpretation.
  • We have world-leading pharmaceutical analysis technology and provide high-quality and large-scale Pharmaceutical Analysis Services.
  • We have an efficient analysis process and fast delivery capability.

CD BioGlyco has extensive experience in the analysis of glycan-related pharmaceuticals and vaccines. We provide our clients with advanced analysis techniques to help them solve their research challenges efficiently. If you are interested in our pharmaceutical analysis services, please feel free to contact us.


  1. Ouyang, H.X.; et al. A simple and selective resonance Rayleigh scattering-energy transfer spectral method for determination of trace neomycin sulfate using Cu2O particle as a probe. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2018, 190: 268-273.
This service is for Research Use Only, not intended for any clinical use.

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