CD BioGlyco provides a variety of glycobiology-related microarray analysis services. We have rich experience in microarray analysis. Using our powerful technology platform and data analysis methods, we guarantee the success of our customers' project. We have confidence to be your essential research assistant at every moment.

Background

Oligonucleotide arrays are the technology used to study cellular events and provide information about mRNA expression levels under various conditions. However, the level of mRNA expression is not always correlated with protein abundance. In addition, analysis of mRNA expression and DNA sequence cannot provide information about its protein. To this end, researchers have developed protein microarrays. Protein microarray can be used as a highly sensitive tool for detecting antibodies in serum. Monitoring the antibody titers of patients against specific antigens is increasingly used to diagnose a variety of diseases, including autoimmune diseases, cancer, infectious diseases and allergies, etc. In addition, protein microarrays can simultaneously measure several biomarkers.

Glycosylation is the most common post-translational modification in proteins, and abnormal glycosylation is usually related to pathological conditions. The up-regulation of unique short aberrant glycosylation on proteins may introduce new glycoprotein epitopes that can elicit autoantibodies due to lack of tolerance. In the past few decades, autoantibody responses to tumor-associated antigens have received extensive attention due to their potential as biomarkers for the early detection of cancer. In addition to using them as biomarkers, the identification of antibody targets can also reveal specific candidate vaccines, such as viruses and cancer vaccines. For this reason, the use of glycoprotein microarray is becoming more prevalent.

Fig 1. Design and experimental workflow for glycoprotein microarray (Pan, L.; et al. 2016)

Service

The general process of CD BioGlyco's glycoprotein microarray analysis is as follows.

First synthesize glycopeptides and use HPLC to efficiently purify glycopeptides, then fix them on microarray slides. In some cases, glycopeptides are modified after synthesis by the enzymes such as glycosyltransferases in solution or in situ on microarray slides. Then the constructed glycoprotein microarray is incubated with samples that may interact with glycoprotein, and finally the binding is detected by fluorescence detection or label-free methods (mass spectrometry or surface plasmon resonance imaging).

Applications

• Identification of biomarkers for cancer and autoimmune disease
• Related vaccine development
• Protein-protein interaction research
• Screening of enzyme substrate specificity

Advantages of Us

• Advanced technology platform and data processing methods
• Measure several sets of data at the same time
• High sensitivity, reproducibility, and adaptability
• Customized service

CD BioGlyco's advanced experimental equipment and well-trained research personnel provide guarantee for customers' glycan-related microarray analysis services. With our high-quality services, we have won praise from customers all over the world.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

Reference:

1. Pan, L.; et al. Three-dimensionally functionalized reverse phase glycoprotein array for cancer biomarker discovery and validation. J. Am. Chem. Soc. 2016, 138(47): 15311-15314.