How much sample is required for the analysis?

We are able to perform a complete analysis with a minimal amount of sample, often in the microgram range. The exact amount depends on the nature of your sample and the planned experiment. We encourage you to discuss your specific needs with our team.

What if the glycans clients are interested in aren't on your array?

Our array is built with a diverse set of biologically relevant glycans. If your target is not included, please reach out to our team to discuss the possibility of a custom array or alternative services.

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100 N-Glycan Microarray

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Overview

100 N-glycan microarray service of CD BioGlyco helps researchers investigate the binding properties of proteins, antibodies, cell cultures, bacteria or other potential biological samples to an array of 100 basic N-glycans. The glycan microarray platform established by our company ensures that we provide customers with high-quality scientific services to accelerate their research.

Introduction

N-linked protein glycosylation is a commonly occurring co-translational and post-translational modification and it is estimated that the majority of all secreted proteins are N-glycosylated. N-glycosylation modifications play an important role in the proper folding, functional localization, and intracellular transport of proteins. In mammals, N-glycosylation is important for the regulation of embryonic development, cancer development, and immune defense. In plants, N-glycosylation is significant for many physiological processes such as cell wall formation, reproductive development and defense against pathogenic bacteria invasion.

Complex N-glycans are involved in a wide range of cellular processes in mammals, especially in intercellular interactions and immune cell functions, so they are generally considered by researchers as the preferred glycan candidates for the investigation of carbohydrate-binding-vaccines like HIV broadly neutralizing antibodies (bNAb). The basic N-glycan structures offered in our 100 N-glycan microarray could help researchers understand the binding determinants underlying antibodies or other proteins of interest.

Key Technologies

Our service for 100 N-glycan microarrays employs fundamental advanced technologies ensuring exceptional reliability and analytical performance. Central to this platform, a curated collection of one hundred chemically identified N-glycans spans wide structural heterogeneity—incorporating high-mannose configurations, hybrid architectures, and complex-type glycoforms. Multiple structures demonstrate biological significance. These glycans undergo exact deposition upon functionalized glass slides, yielding microarrays of high density. Array stability and reproducible functioning derive from printing and immobilization through proprietary methods. This permits consistent, sensitive measurement of interactions between glycans and proteins—critical for elucidating diverse biological processes.

Mapping Glycan Diversity, One Spot at a Time

CD BioGlyco provides customers with microarray service for 100 N-glycans, including 81 complex and hybrid N-glycans, 10 high-mannose N-glycans and 9 Neu5Gc-N-glycans. The service we provide include but are not limited to:

Traditional glycan microarrays. This method requires very small amounts of test glycans to provide detailed information on the effect of glycan modification on GBP recognition.
Shotgun glycan microarrays, a functional glycomics approach, provide for the characterization of the actual glycan ligands separated from natural sources for a specific GBP.
Microbial glycan microarrays to examine the specificity of host factors directly involved in host-pathogen interactions.

Workflow

Sample Preparation

Your biological samples (e.g., purified proteins, antibodies, or sera) are prepared and labeled with a fluorescent tag. This step is critical to ensure accurate detection of binding events.

Array Incubation

The labeled sample is incubated on the 100 N-glycan microarray slide. During this step, any glycan-binding proteins in your sample will bind specifically to their corresponding glycans on the array.

Washing and Scanning

Unbound proteins are washed away, and the array is scanned using a high-sensitivity fluorescent microarray scanner. This captures the binding signal, generating a raw data image.

Data Analysis

The raw image data is then processed using specialized software. We quantify the binding intensity for each glycan spot, normalize the data, and perform statistical analysis to identify significant binding interactions.

Comprehensive Report

We deliver a detailed report that includes the raw data, processed results, and a clear visualization of the binding profile. Our experts are available to help you interpret the findings and discuss their biological implications.

Our workflow. (CD BioGlyco)

Publication Data

DOI.: 10.3389/fchem.2019.00833

Journal: Frontiers in Chemistry

Published: 2019

IF: 4.2

Results: This review details the development and immunological applications of glycan microarrays as essential tools for identifying glycan recognition by immune proteins. The authors describe the chemical strategies for constructing these microarrays, particularly covalent attachment methods using bifunctional linkers like AEAB and F-MAPA to immobilize diverse glycans while preserving structural integrity. They highlight how glycan microarrays enable high-throughput profiling of glycan-binding proteins (GBPs) such as siglecs and galectins, revealing their specificities for sialylated or LacNAc-containing glycans. The review emphasizes the role of these arrays in immunological discoveries, including identifying natural ligands for endogenous immune receptors, characterizing pathogen-host interactions (e.g., influenza, rotavirus), and mapping the human anti-glycan antibody repertoire. Innovations like DNA-coded arrays, Luminex bead arrays, and cell-based platforms are also discussed as emerging methods to enhance throughput and biological relevance. Overall, glycan microarrays are portrayed as indispensable for deciphering glycan-mediated immune mechanisms and advancing diagnostic and therapeutic research.

Application

Determination of N-glycans binding partners in various biological samples
Rapid profiling of N-glycan binding properties of proteins
Providing important information for host immunity and infectious disease
Understanding GBP glycan interactions involved in immunity

Advantages

Our 100 N-glycan microarray offers an unparalleled combination of breadth and specificity, covering a diverse range of N-glycan structures critical for biological research.
The high-throughput nature of our platform allows for the rapid and simultaneous analysis of multiple samples, significantly reducing project timelines and costs.
Our platform demonstrates its sensitivity and reproducibility in identifying glycan-binding partners.

Frequently Asked Questions

Associated Services

Our 100 N-glycan microarray decodes functional glycan signatures across biological systems—revealing critical targets in cancer, infection, and immune regulation. To transform these insights into precision interventions, we offer N-Glycosylation Inhibitor Development Services that disrupt glycan biosynthesis or interactions through complementary strategies.

CD BioGlyco offers a range of glycan microarray analysis services. These help our customers speed up their scientific research and obtain reliable data by using our microarray platform to perform highly parallel glycan expression level analysis. If you need our services, please contact us for further consultation.

Reference:

Gao, C.; et al. Glycan microarrays as chemical tools for identifying glycan recognition by immune proteins. Frontiers in chemistry. 2019, 7: 833. (Open Access)