Disaccharides play important roles in mediating biological processes, food sweeteners, and the healthcare products field. The interest of scientists in disaccharide synthesis has greatly increased. CD BioGlyco has abundant experience in customizing disaccharide synthesis services according to customers' research needs. We expect to become your research assistant in the field of glycobiology.

Introduction

Disaccharides are glycosides produced by condensation and dehydration of hydroxyl groups on two identical or different monosaccharides. Sucrose, maltose, and lactose are three common disaccharides. Glycosidic bonds can be formed between any hydroxyl groups on the component monosaccharides. Therefore, even if two sugars have the same composition (e.g., glucose), different bond combinations (regiochemistry) and stereochemistry (α- or β-) can result in disaccharides as diastereoisomers with different physical and chemical properties. Therefore, the custom synthesis of disaccharides with complex structures is important for studying their functions in the fields of cosmetics, biotechnology, pharmaceuticals, and food production.

The traditional preparation method of disaccharides is acid hydrolysis and purification of natural glycan. However, it has the disadvantages of the limited supply of corresponding natural glycans, complex acidolysis products, and high purification costs. People are more inclined to use a suitable enzyme for the synthesis of disaccharides such as glycoside phosphorylase. With the development of enzyme catalysis technology, scientists can produce value-added disaccharides through an in vitro synthetic enzymatic platform, exhibiting great potential on an industrial scale.

Fig.1 Synthesis of functional disaccharide in vitro by multi-enzyme system. (Sun, 2020)

Synthesis Methods

CD BioGlyco has established a variety of methods to provide customers with customized synthesis services of disaccharides. Our methods include but are not limited to:

• We adopted an artificial strategy for producing disaccharides through the construction of an in vitro synthetic enzymatic platform. This method not only reduces the cost but also ensures the purity of the product.
• Protein engineering of glycoside hydrolases (GHs) was used to eliminate hydrolytic activity and improve synthetic activity for the synthesis of disaccharides.
• We synthesize disaccharides from monosaccharides through reverse hydrolysis reaction by improving the hydrophobicity of the entrance to the active site or acceptor subsite.

Advantages of Us

• Effective multi-step synthesis
• Customized route design
• Advanced facilities and well-trained researchers
• Cutting-edge synthesis method

CD BioGlyco has extensive experience in custom synthesis of disaccharides. Our advanced experimental platform, professional research team, and optimized synthesis process allow us to provide our clients with high-quality disaccharide synthesis services. If you are interested in our services, please feel free to contact us for more details.

References:

1. Sun, S.; et al. Construction of an artificial in vitro synthetic enzymatic platform for upgrading low-cost starch to value-added disaccharides. Journal of Agricultural and Food Chemistry. 2021, 69(1):302-314.
2. Niu, K.; et al. A novel strategy for efficient disaccharides synthesis from glucose by β-glucosidase. Bioresour. Bioprocess. 2020, 7,45.