Overview

Disaccharides play important roles in mediating biological processes, food sweeteners, and the healthcare products field. The interest of scientists in disaccharide synthesis has greatly increased. CD BioGlyco has abundant experience in customizing disaccharide synthesis services according to customers' research needs. We expect to become your research assistant in the field of glycobiology.

Key Technologies

Protein engineering; Reverse hydrolysis

Precision-Bonded Sugars, Perfectly Tailored

Disaccharides are glycosides produced by condensation and dehydration of hydroxyl groups on two identical or different monosaccharides. Sucrose, maltose, and lactose are three common disaccharides. Glycosidic bonds can be formed between any hydroxyl groups on the component monosaccharides. Therefore, even if two sugars have the same composition (e.g., glucose), different bond combinations (regiochemistry) and stereochemistry (α- or β-) can result in disaccharides as diastereoisomers with different physical and chemical properties. Therefore, the custom synthesis of disaccharides with complex structures is important for studying their functions in the fields of cosmetics, biotechnology, pharmaceuticals, and food production.

The traditional preparation method of disaccharides is acid hydrolysis and purification of natural glycan. However, it has the disadvantages of the limited supply of corresponding natural glycans, complex acidolysis products, and high purification costs. People are more inclined to use a suitable enzyme for the synthesis of disaccharides such as glycoside phosphorylase. With the development of enzyme catalysis technology, scientists can produce value-added disaccharides through an in vitro synthetic enzymatic platform, exhibiting great potential on an industrial scale.

Synthesis Methods

CD BioGlyco has established a variety of methods to provide customers with customized synthesis services of disaccharides. Our methods include but are not limited to:

• We adopted an artificial strategy for producing disaccharides through the construction of an in vitro synthetic enzymatic platform. This method not only reduces the cost but also ensures the purity of the product.
• Protein engineering of glycoside hydrolases (GHs) was used to eliminate hydrolytic activity and improve synthetic activity for the synthesis of disaccharides.
• We synthesize disaccharides from monosaccharides through reverse hydrolysis reaction by improving the hydrophobicity of the entrance to the active site or acceptor subsite.

Publication Data

Workflow

Applications

• Custom-synthesized disaccharides serve as precise molecular probes to investigate carbohydrate-protein interactions, enabling researchers to elucidate mechanisms such as lectin binding specificity, cell signaling pathways, and pathogen-host adhesion processes.
• These tailored disaccharides are instrumental in designing glycosidase inhibitors for metabolic diseases, developing glycomimetic drugs that target G-protein-coupled receptors (GPCRs) or immune checkpoints.
• Chemically modified disaccharides bearing fluorescent tags or isotopic labels enable metabolic glycan labeling in live cells, advancing non-invasive imaging techniques to track glycosylation patterns in diseases

Advantages

• We employ engineered in vitro enzymatic systems that precisely control glycosidic bond formation (α/β linkages and regiochemistry), ensuring high-purity disaccharides while avoiding the limitations of traditional acid hydrolysis methods.
• We develop tailored synthesis routes for complex diastereoisomers, leveraging reverse hydrolysis or phosphorylase-based methods to meet specific structural requirements (e.g., drug-conjugate precursors or diagnostic probes).
• Our enzymatic platforms reduce production costs by using low-cost substrates (e.g., glucose) and minimizing purification steps, enabling industrial-scale synthesis without compromising purity.

Frequently Asked Questions

Associated Services

CD BioGlyco has extensive experience in custom synthesis of disaccharides. Our advanced experimental platform, professional research team, and optimized synthesis process allow us to provide our clients with high-quality disaccharide synthesis services. If you are interested in our services, please feel free to contact us for more details.