Sialylated Oligosaccharides Microarray Assay
Unlocking the Secrets of Sialylated Oligosaccharides Microarray with Precision
Sialylation is a form of glycosylation, and sialylated oligosaccharides are involved in the regulation of a variety of physiological activities. For example, it stabilizes protein structure, prolongs the half-life of glycoproteins, and participates in cellular recognition. At CD BioGlyco, we have constructed a Glycan Display Platform and Sialic Acid Microarray, and based on these, we further propose a sialylated oligosaccharide Microarray Assay.
We have already developed a comprehensive and advanced sialylated oligosaccharide microarray construction service and the standard process is shown below. Besides, we also would like to work with our clients to develop more custom microarrays.
- Oligosaccharide library establishment
We establish a library of sialylated oligosaccharides based on natural sialylated oligosaccharides, biosynthesized, chemically synthesized, and enzymatically synthesized sialylated oligosaccharides. - Sample immobilization
We use non-covalent methods to immobilize sialylated oligosaccharides on the surface of solid-phase carriers. To overcome the hydrophilicity of the oligosaccharides, we attach a lipid molecule to the sialylated oligosaccharides by reductive amination to increase the hydrophobicity of the oligosaccharide probes. Finally, it is noncovalently adsorbed onto the solid-phase carrier (e.g., nitrocellulose membrane) by hydrophobic forces. - Detection and processing of markers
We provide both fluorescent and radiolabeled markers. Detection methods include matrix scanners, mass spectrometry, and liquid chromatography.
Fig.1 Process of sialylated oligosaccharide microarray construction. (CD BioGlyco)
Our construction of sialylated oligosaccharide microarrays immobilizes multiple sialylated oligosaccharide groups on a single chip. It simultaneously performs multi-parameter assays on multiple samples under the same conditions. Specific assays include but are not limited to.
- Interaction of sialylated oligosaccharides with monosaccharides as well as with polysaccharides
- Specific binding between oligosaccharides and proteins, analysis of glycan binding properties of specific proteins
- Determination of affinity oligosaccharides for quantitative binding of proteins
- Screening of functional glycans
Publication
Technology: Fluorescein-streptavidin coupling method
Journal: Glycoconjugate Journal
IF: 3.0
Published: 2008
Results: Authors in this paper developed glycan microarrays that can be used for high-throughput receptor-specific screening of various recombinant salivaryltransferases. Cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-Neu5Ac) is synthesized chemically by chemoenzymatic synthesis of N-acetylneuraminic acid (Neu5Ac) at position 9 to produce CMP-9Biot-Neu5Ac biotinylation. The activated glycan nucleotide was used as a donor substrate for various mammalian sialyltransferases that transfer biotinylated salivary acid simultaneously to glycan acceptors immobilized on microarray slides. The biotinylated glycans were detected with a fluorescein-streptavidin coupling to generate specificity maps for each enzyme, both confirming previously known specificities and revealing additional specificity information. In addition, the method permits rapid generation of enzyme-specific information and can be used to synthesize new carbohydrate compounds and expand glycan array compound libraries.
Applications
- Sialylated oligosaccharide microarrays can be used to determine the interactions between sialylated oligosaccharides and biomolecules such as proteins, cells, and cell lysates in a variety of physiological activities (cell signaling, cell proliferation, tumor transformation, growth, and metastasis, etc.).
- Sialylated oligosaccharide microarrays can be used to further the development of glycomics.
- Sialylated oligosaccharide microarrays can be used to characterize the structure of glycans, sequence glycans, and detect specific binding between glycans and proteins.
- Sialylated oligosaccharide microarrays can help advance the development of diagnostic tools based on microarray technology.
Advantages
- We construct sialylated oligosaccharide microarrays with high stability.
- We have a professional glycan display platform and provide custom Microarray Printing services according to clients' needs.
- We provide comprehensive data analysis about sialylated oligosaccharides.
CD BioGlyco has established a powerful glycan display platform to provide advanced custom glycan microarray assay and timely pre-sales, and after-sales services to clients all over the world. Our service team covers experts in the field of glycobiology, ensuring that the services we provide are of high quality and accuracy. If you are interested in our glycan microarray technology, please feel free to contact us and we will reply promptly.
References
- Pomin, V.H.; Wang, X. Synthetic oligosaccharide libraries and microarray technology: a powerful combination for the success of current glycosaminoglycan interactomics. ChemMedChem. 2018, 13(7): 648-661.
- Blixt, O.; et al. Glycan microarrays for screening sialyltransferase specificities. Glycoconjugate Journal. 2008, 25: 59-68.
