Enzymatic Release

Studying the detailed structural and functional characterization of different types of glycans is essential to understand the mechanism of various biological activities and the pathology of several diseases. CD BioGlyco has been trying to develop simple and effective enzymatic methods to release glycans from complex biological samples. We expect to be your research assistant in the field of glycobiology.

Background

Enzymatic Release

Glycosylation is an important way of post-translational modification of proteins, which has a variety of biological functions, such as immune recognition, signal transduction, cell adhesion, and tumorigenesis. Therefore, the development of effective methods to release glycans from the protein backbone is of great significance to the study of their structure and function.

In contrast to chemical methods of glycan release, enzymatic release can be carried out with minimum instructions in any laboratory. In the past two decades, more and more enzymes including endoglycosidases and glycosidases have been reported to release glycans from glycoproteins under mild conditions. The endoglycosidase endo-β-N-acetylglucosaminidase H (Endo H), peptide-N4-(acetyl-β-glucosaminyl) asparagine amidase F (PNGase F), and PNGase A are the most preferred endoglycosidases, N-glycans can be conveniently and non-selectively released from glycoproteins using these enzymes.

Release Strategies

CD BioGlyco mainly focuses on the release of N-glycan and established effective enzymatic methods for the nonspecific release of glycan chains. The services we can provide include but are not limited to:

  • Releasing N-glycans from glycoprotein with non-specific enzyme Pronase E instead of traditional PNGase F.
  • Releasing N-glycans by two protocols: one using trypsin to destroy the protein structure, while the other one using detergents before PNGase F digestion. Thereafter, reverse-phase cartridges are used to isolate N-glycans from peptides and carbograph cartridges are used for desalting before final derivatization with fluorescent label 2AB.

Enzymatic Release

Applications

  • Research on the interaction between glycan chains and proteins
  • Research on the complicated structure of glycans
  • Discovery of glycan chains related targets of some drugs
  • Discovery of glycan chains biomarkers of major diseases

Advantages of Us

  • Professional research team and well-trained experimenters
  • Systematic experimental platform and advanced technical approaches
  • Efficient technical service and competitive price
  • Timely response after-sales service

CD BioGlyco focuses on teamwork and uses first-class experimental platforms to provide global biotechnology companies and scientific research institutions with efficient and high-quality services related to glycobiology. Our fast and effective solutions will greatly accelerate the progress of our clients' projects. Please contact us directly for more detailed information if you need scientific assistance.

References:

  1. Xu, S.; et al. Pronase E digestion of N-glycans in glycoprotein and its fluorescent derivatives analysis by HPLC-ESI/MS. Chemical Journal of Chinese Universities-Chinese Edition. 2010, 31(10):1992-1998.
  2. Grunow, D.; Blanchard, V. Enzymatic release of glycoprotein N-glycans and fluorescent labeling. InPost-Translational Modification of Proteins. 2019, 43-49.
This service is for Research Use Only, not intended for any clinical use.

About Us

CD BioGlyco is a world-class biotechnology company with offices in many countries. Our products and services provide a viable option to what is otherwise available.

Contact Us

Copyright © CD BioGlyco. All rights reserved.