Lactulose is a synthetic disaccharide containing galactose and fructose molecules, which is chemically similar to lactose. Lactulose is not digested and absorbed in the gut, while improves gut health by promoting the growth of beneficial flora for example bifidobacteria and lactic acid bacteria. Lactulose is decomposed by bacteria into organic acids (for example, acetic acid, propionic acid, and lactic acid) when it enters the colon, which can regulate the intestinal acid-base balance, increase the acidic environment, and reduce the growth of harmful bacteria. Due to its special physical and chemical properties, lactulose can be applied in the medical field as a mild laxative and bowel regulator for the treatment of constipation or other problems related to intestinal dysfunction. Moreover, it can also be applied in the treatment of certain liver diseases, which can help reduce the concentration of ammonia in the blood and improve liver function.
Fig.1 Basic structure of lactose and lactulose. (Silveira, et al., 2015)
Fig.2 Featured lactulose analysis services. (CD BioGlyco)
Paper Title: Simultaneous determination of lactulose and lactose in conserved milk by HPLC-RID
Journal: Journal of Chemistry
Results: The author used HPLC-RID to simultaneously determine the lactulose and lactose contents of dairy products, and used the protein precipitation method to pretreat the milk samples. Prevail Carborather ES was used as the chromatographic column, acetonitrile: water as the mobile phase, and isocratic HPLC analysis was performed under the conditions. The results showed that the retention times of lactulose and lactose in milk were 13.6 and 17.2 minutes respectively, they were completely separated, the method was sensitive and stable, and the precision was good.
Fig.3 Lactose-lactulose chromatogram by HPLC-RID. (Silveira, et al., 2015)
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