Enoxaparin Sodium Analysis Service

Enoxaparin Sodium Analysis Service

Precision Meets Excellence in Enoxaparin Sodium Analysis

Enoxaparin sodium is a low molecular weight heparin that is effective in the prevention of deep vein thrombosis or pulmonary embolism. CD BioGlyco has world-leading technology in the field of Pharmaceutical Analysis and provides cutting-edge enoxaparin sodium analysis services to every client.

Efficient and Precise Enoxaparin Sodium Analysis at CD BioGlyco

  • Identification
    • Spectra: We offer ultraviolet-visible (UV-Vis) spectroscopy for the identification of enoxaparin sodium. Compare the spectrograms of the enoxaparin sodium sample and standard, if both spectrograms are consistent, then this sample is enoxaparin sodium.
    • Nuclear magnetic resonance carbon spectra (13C NMR): The 13C NMR spectra of the sample to be tested and the enoxaparin sodium standard are tested separately. If the two spectra show the same data, the sample is enoxaparin sodium.
    • Molecular weight distribution and weight-average molecular weight (Mw): Sample solution weight-mean Mw is tested using gel permeation chromatography (GPC). Under normal conditions, the Mw distribution of enoxaparin sodium results in M20000 is between 12.0% and 20.0%, M2000-8000 is between 68.0% and 82.0%, and M8000 is 18.0%.
  • Benzhydrol content
    We dissolve and dilute enoxaparin sodium using a buffer, and then test it using liquid chromatography (LC).
  • Sodium content
    The absorbance of the sample solution at a specific wavelength is determined using atomic absorption spectroscopy (AAS) to obtain the amount of sodium in the sample solution.
  • pH
    We test the pH of enoxaparin sodium by dissolving it in water. Normally, it has a pH between 6.2 and 7.7.
  • Loss on drying
    The enoxaparin sodium is dried in a vacuum at high temperature and then its weight loss is calculated. Normally, its weight loss on drying does not exceed 10.0%.
  • Anti-factor IIa activity
    The enoxaparin sodium is dissolved in water and then diluted with a buffer. Next, the absorbance of the enoxaparin sodium solution is calculated. The mean value and the potency of enoxaparin sodium in IIa activity are calculated after several repetitions of the test. In turn, the anti-factor IIa activity of enoxaparin sodium is expressed.

In addition, enoxaparin sodium is also used in pharmaceutical applications as an injection. CD BioGlyco also offers comprehensive enoxaparin sodium injection analysis services. Most of the analysis methods for enoxaparin sodium injection are the same as those for enoxaparin sodium. However, there are differences in its identification and pH values from enoxaparin sodium.

  • Identification of enoxaparin sodium injection
    Put the sample in a glass test tube, add water and a solution of ichthyosperm sulfate, and mix. If a milky white precipitate appears, the sample is enoxaparin sodium injection.
  • pH of enoxaparin sodium injection
    Dilute enoxaparin sodium injection with water and then we test its pH. It has a pH between 5.5 and 7.5.

Fig.1 Enoxaparin sodium analysis services. (CD BioGlyco)Fig.1 Enoxaparin sodium analysis services. (CD BioGlyco)

Publication Data

Technology: High-performance liquid chromatography (HPLC)

Journal: ACS Omega

IF: 4.1

Published: 2023

Results: The authors developed an HPLC method to validate enoxaparin sodium. All injection volumes of the HPLC samples were 20 μL and the chromatographic separation was done at isocratic mode. A refractive index (RI) detector was used to analyze the samples and a specific peak for enoxaparin sodium was able to be observed as early as 5:56 min. Such a newly developed method can be effectively used for the precise determination of enoxaparin sodium in injectable formulations. At the same time, it is cost-effective, requires less time for each investigation, and consumes a smaller amount of sample.

Fig.2 Process of HPLC validation of enoxaparin sodium. (Yousaf, et al., 2023)Fig.2 Process of HPLC validation of enoxaparin sodium. (Yousaf, et al., 2023)


  • We provide our clients with reliable Pharmaceutical and Biological Analysis that ensures accurate and efficient results.
  • We have cutting-edge pharmaceutical analysis technologies to ensure comprehensive pharmaceutical analysis.
  • We provide custom pharmaceutical analysis solutions according to our client's needs, ensuring that we meet their various research requirements.

CD BioGlyco provides efficient and accurate pharmaceutical analysis services that are customized to meet the needs of our clients. All of our pharmaceutical analysis solutions undergo rigorous quality testing. If you would like to know more about pharmaceutical analysis, please feel free to contact us.


  1. Yousaf, M.H.; et al. Development and validation of RP-HPLC method for the determination of enoxaparin sodium in dry injection formulation. ACS Omega. 2023, 8(47): 44988-44994.
This service is for Research Use Only, not intended for any clinical use.

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