CD BioGlyco is a world-leading independent biotechnology company, focusing on high-quality innovative products and services related to glycobiology. We have established an advanced and specialized platform to help our customers efficiently synthesize high purity phosphatidylinositol (PI) according to their different research demands.

Properties and Biosynthesis of PI

PI is an essential acidic phospholipid, which exists in the membrane of mammalian cells and chromatin. PI is mainly composed of two parts: 1,2-diacylglycerol phosphate and inositol. The inositol ring of PI can be phosphorylated at three positions alone or in combination to produce seven derivatives. PI plays important roles in cell morphology, metabolic regulation, signal transduction, and various physiological functions. In addition, the PI can be phosphorylated to form PI phosphate (PI-4-P), PI diphosphate (PIP₂), and PI triphosphate (PIP₃). All PI-based lipids are known as inositides, sometimes phosphoinositol.

The de novo biosynthesis of PI starts from the precursors 3-phosphoglycerol or dihydroxyacetone phosphate and the process only occurs in the endoplasmic reticulum (ER). These molecules undergo two sets of acylation under the action of acyltransferase to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. The synthesis of PI in the laboratory is catalyzed by phosphatidylinositol synthetase, involving CDP-diacylglycerol and L-inositol.

Fig.1 Synthesis of PI at the ER and of cardiolipin (CL) in mitochondria. (Blunsom, 2020)

Services

As a leading resource provider in the glycobiology field, in addition to providing customers with the synthesis services of a wide range of PI and their derivatives, CD BioGlyco also focuses on synthetic research of glycosylphosphatidylinositol (GPI), which acts as a membrane anchor for many cell surface proteins. We have explored important methods of chemical synthesis combined with enzyme-catalyzed reaction to obtain GPI and GPI anchored proteins with a clear structure and high purity. We are able to provide the synthesis of different kinds of GPI such as the synthesis of GPI containing non-acylated inositol and GPI containing 2-acylated inositol.

Applications

• Study on phosphoinositide signal pathway
• Study on biological functions of PI
• Research on PI biosynthesis pathway

Advantages of Us

• Flexible and efficient customization platform
• Experienced and well-trained technicians
• Optimal experimental conditions
• High flexibility and cost-effectiveness
• Detailed after-sales services

With a first-class experimental platform and professional research teams, CD BioGlyco is committed to ensuring that every process providing the highest level of service and satisfaction. Our comprehensive custom synthesis services have been recognized in the field of glycobiology. Please do not hesitate to contact us if you have a scientific need for custom PI synthesis.

Reference:

1. Blunsom, N.J.; Cockcroft, S. CDP-diacylglycerol synthases (CDS): gateway to phosphatidylinositol and cardiolipin synthesis. Frontiers in Cell and Developmental Biology. 2020, 7;8:63.