Anti-glycan Polyclonal Antibody Development

CD BioGlyco has professional antibody development teams and first-class technology platforms, which can provide customers with unparalleled antibody development services and complete after-sales service for research projects. We have confidence to be your essential research assistant in the field of glycobiology.

Background 

Anti-glycan Polyclonal Antibody Development

Antibody refers to the protective protein produced by the body due to the stimulation of antigen. It has a binding specificity with a specific antigen. Monoclonal antibodies (mAbs) are produced by B cells and only recognize the same epitope of the antigen, giving mAbs high-affinity characteristics. Polyclonal antibodies (pAbs) are heterogeneous mixtures, usually produced by different B cell clones in the body, and they can recognize and bind to many different epitopes of a single antigen.

Glycans (or carbohydrates) are the main surface components of cells (such as red blood cells, immune cells and microorganisms) and participate in a variety of life activities. At present, more anti-glycan antibodies are used in scientific research and clinical trials. Compared with mAbs, anti-glycan pAbs can significantly shorten the production cycle and reduce production costs. It has comprehensive effects, and important functions such as neutralizing antigens, immune regulation, complement-dependent cytotoxicity (CDC), antibody-dependent cell-mediated cytotoxicity (ADCC), high stability, and can tolerate pH changes and small changes in glycan antigens (such as glycosylation heterogeneity). However, anti-glycan polyclonal antibodies are prone to cross-reactions, and differences between batches are likely to occur during the production process.

Anti-glycan Polyclonal Antibody Development

Solutions

At CD BioGlyco, we pay attention to every detail in antibody development and strive for perfection in every process, evidenced by our many years of successful experience. We provide numerous species for the production of anti-glycan polyclonal antibodies, including rabbits, goats, sheep, chickens, mice, rats and hamsters, etc. Goats/sheep are the preferred host for mass production of polyclonal antibodies because they can produce a strong immune response to most antigens, and the amount of serum produced by goats can reach 7-8 times that of rabbits. The most cost-effective hosts are chickens and rabbits, which can provide high-titer, high-affinity serum in a short time.

CD BioGlyco integrates chemistry and biology perspectives into antibody development. At the same time, first-class project management and purification strategies can ensure the high success rate of polyclonal antibody production, and considerable amounts of polyclonal antiserum can be obtained in a short period (4-6 weeks) with minimal investment.

Applications

  • Monitor and detect carbohydrate expression
  • Study glycans' mechanism of action
  • Agonist and antagonist screening
  • Development of glycan/glycoprotein purification methods
  • Discovery of disease-related carbohydrates

Advantages of Us

  • Production procedures that comply with regulations
  • High yield and high specificity
  • Expert advice at every step of the experimental process
  • Multiple advanced technology platforms
  • Systematic specificity evaluation protocol

CD BioGlyco provides a complete anti-glycan polyclonal antibody development program, and our experts can customize experimental scheme according to customers' scientific research projects. We offer first-class and cost-effective polyclonal antibodies services to help customers accelerate their project.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

Reference:

  1. Wootla, B.; et al. Polyclonal and monoclonal antibodies in clinic. Methods Mol Biol. 2014: 79-110.
This service is for Research Use Only, not intended for any clinical use.

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About Us

CD BioGlyco is a world-class biotechnology company with offices in many countries. Our products and services provide a viable option to what is otherwise available.

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