2023 Meeting of the Society for Glycobiology
November 5–8, 2023
Hilton Waikoloa Village Resort, on the big island of Hawaii

High-Throughput Screening for Glycan-Binding Proteins

Biological Significance of Glycan-Binding Proteins

Glycan-binding proteins are proteins that specifically recognize and bind to carbohydrates on the surface of cells, tissues, or molecules, and some of them also function as lectins. They are involved in a wide range of biological processes. Different types of these proteins have different carbohydrate-binding domains (CBDs) that bind specific types of glycosylated substances. Some examples of glycan-binding proteins include galectins, selectins, and C-type lectins. In conclusion, glycan-binding proteins are of great importance in multiple aspects of cellular, molecular, and physiological processes. The study of their functions and regulatory mechanisms is important for a deeper understanding of fundamental questions in glycobiology and for developing disease-related research. High-throughput screening (HTS) can be used to predict small molecule compounds with binding sites and binding affinities to glycan-binding proteins. This helps researchers understand the molecular mechanisms of interactions with glycan-binding proteins and provides clues for designing new drugs or therapeutic strategies.

Procedure for the screening of glycan-binding proteins by miniature pig kidney N-glycan-immobilized beads and mass spectrometry.Fig.1 Procedure for the screening of glycan-binding proteins by miniature pig kidney N-glycan-immobilized beads and mass spectrometry. (Kim, et al., 2008)

HTS of Glycan-Binding Proteins

CD BioGlyco is focused on the research of high-quality glycan-binding proteins for drug development. We are committed to combining its proprietary technology with innovative science to provide our clients with high-quality glycan-binding proteins HTS services. Our services encompass a wide range of offerings, including but not restricted to:

  • HTS of glycan-binding proteins by Miniature Pig Kidney N-Glycan-Immobilized Beads
    This technology selectively immobilizes target glycosyl groups on the surface of beads, so that only proteins with high affinity for beads would bind to them, allowing for a highly selective screening.
  • HTS of glycan-binding proteins by Glycan Microarrays
    Each glycan on the glycan microarray has its location, and glycan-binding proteins will only bind to specific types of glycans, thus improving the selectivity and being more cost-effective than traditional protein-binding assays.
  • HTS of glycan-binding proteins by 19F-Labeled Probes
    19F is a unique NMR spectrum with high sensitivity to chemical shifts and a strong correlation with its surrounding chemical environment, thus contributing to fast, accurate, and high-throughput detection of molecule-molecule interactions.

HTS technologies applied to glycan-binding proteins.Fig 2. HTS technologies applied to glycan-binding proteins. (CD BioGlyco, 2023)

Applications

  • Drug development
  • Research on glycomics
  • Preparation of glycol-based vaccines

Advantages of Us

  • Multidimensional research
  • Efficient screening
  • Customizable services

CD BioGlyco is committed to providing clients with the complete solution for glycosylated protein analysis, including HTS services of glycan-binding proteins, to help clients achieve optimal research results and commercial benefits. If you are interested in our services, please feel free to contact us for more details.

Reference

  1. Kim, Y.G.; et al. High-throughput screening of glycan-binding proteins using miniature pig kidney N-glycan-immobilized beads. Chemistry & biology. 2008, 15(3): 215-223.
This service is for Research Use Only, not intended for any clinical use.

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