GPI-PET is an enzyme involved in the modification of GPI-anchored proteins by phosphoethanolamine. During the synthesis of mammalian GPI anchors, three different GPI-PETs add phosphoethanolamine (P-EthN) groups to each of the three Man residues in the GPI glycan core. This step is critical for GPI anchoring because the P-EthN group provides the link between the glycolipid and the protein. CD BioGlyco has one-stop Glycosylation Inhibitor Development solutions, and we provide you with an efficient and flexible GPI-PET inhibitor development service.
CD BioGlyco provides high-quality GPI-PET inhibitor development services in multiple pathways.
CD BioGlyco provides efficient GPI-PET inhibitor evaluation services to verify their inhibitory efficacy and selectivity.
In addition, CD BioGlyco provides mature GPI-PET inhibitor production service. We select appropriate synthesis routes, reaction conditions, catalysts, solvents, and other process conditions to optimize the production process of GPI-PMT inhibitors, thereby ensuring that the production process is efficient, repeatable, and has good product yields.
Fig.1 GPI-PET inhibitor development service. (CD BioGlyco)
Technology: Thin-layer chromatography
Results: The authors studied the mechanism of action of 1,10-phenanthroline (PNT) on GPI anchor synthesis. PNT was added to HeLa cells. As its concentration increased, the level of H6 reached a maximum at 100 μM PNT, while H2 detected that higher PNT concentrations resulted in greater H2 accumulation and a parallel decrease in H6. Studies showed that PNT was a novel inhibitor of GPI-anchored synthesis that caused the accumulation of GPI-anchored intermediates that were substrates for GPI-PET, suggesting that these enzymes were targets of PNT.
Fig.2 Dose-dependent inhibition of GPI synthesis by PNT. (Mann & Sevlever, 2001)
CD BioGlyco continues to pay attention to the latest industry research progress and technological innovation. We apply the latest scientific methods to the development of GPI-PET inhibitors and provide you with optimal GPI-PET inhibitor development services. Please feel free to contact us if you would like to get specific development details.