Melibiose Analysis Service at CD BioGlyco

Melibiose is an analog of Trehalose, which is linked by α-1,6-glycosidic bonds to a molecule of glucose and a molecule of galactose. At CD BioGlyco, we have complete Carbohydrate Metabolism analysis solutions and provide melibiose analysis services of different sources for our clients all over the world.

• High-performance liquid chromatography (HPLC) and electrospray ionization mass spectroscopy (ESI-MS)

HPLC is the most commonly used technique for the determination of sugar content in samples. The method shows the advantages of precision, selectivity, and sensitivity. Our detectors include refractive index detection (RID), ultraviolet (UV), and evaporative light scattering detector (ELSD). Meanwhile, melibiose labeling is suitable for detection by UV detector, which improves the sensitivity and accuracy of detection. After HPLC analysis, the samples are analyzed in tandem with ESI-MS for sample characterization, and tandem mass spectrometry is used to attribute and determine the structure of the sugar chain from the fragmentation feature profiles derived from multistage cleavage of the sugar chain.

• LC-MS

LC-MS has realized the simultaneous online detection of different sugar chains of different sizes. We add the internal standard to the comprehensive analysis of the target samples.

• Other methods

Moreover, CD BioGlyco offers nuclear magnetic resonance (NMR), X-ray, and Fourier transform infrared spectroscopy (FTIR) analysis of melibiose. We provide accurate and reproducible analysis services to our clients.

Fig.1 Flowchart of melibiose analysis. (CD BioGlyco)

Publication

Paper Title: analysis of the site-specific myoglobin modifications in the melibiose-derived novel advanced glycation end-product

Technology: HPLC, MS, FTIR.

Journal: International Journal of Molecular Sciences

IF: 6.2

Published: 2022

Results: In the study, myoglobin (MB) glycosylated with mel is analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), showing a mixture of products with different molecular masses and degrees of cross-linking. The mel under dry conditions (MWG) condition favored the generation of products with a higher degree of cross-linking, a richer mel loading, and different antigenic properties.

FTIR analysis showed that the protein structure is significantly altered after glycosylation under both conditions. The differences in physicochemical properties between MWG and conventional glycation condit glycosylation products are confirmed by absorption and fluorescence properties.

Analysis of the spatial distribution within the proteins of known advanced glycation end-products (AGEs) using LC-MS/MS also revealed differences in the antigenic properties of melibiose-derived advanced glycation end-product (MAGE), showing specific MAGE-modified blood proteins. Further studies of the spatial modifications of immunoglobulins and serum albumin are highly correlated with MAGE-modification-associated cancers and diabetic diseases.

Fig.2 Graphical representation of the analytical results of melibiose. (Gostomska-Pampuch, et al., 2022)

Applications

• Melibiose analysis is used to heed the aggregation of α-synuclein to upregulate autophagy to mitigate neurotoxicity in neurodegenerative diseases.
• Melibiose analysis increases dopamine transporters, suppresses oxidative stress, reduces the activation of microglia and astrocytes, and enhances brain antioxidant and autophagy functions as novel targets for drug function development.

Advantages

• Multiple technologies are used for melibiose analysis in a single or combined assay with good reproducibility.
• Our company provides different analytical detection methods based on markers or biological samples.

CD BioGlyco provides high-quality Disaccharide Analysis with a professional team. Moreover, we provide analysis services including Monosaccharides, Oligosaccharides, and polysaccharides. We are capable of providing efficient and reliable services to accelerate our clients' scientific research by shortening the project time. If you are interested in our services, please feel free to contact us.

References:

1. Gostomska-Pampuch, K.; et al. Analysis of the site-specific myoglobin modifications in the melibiose-derived novel advanced glycation end-product. International Journal of Molecular Sciences. 2022, 23(21): 13036.
2. Watson, A.; et al. NMR solution geometry of saccharides containing the 6-O-(α-D-glucopyranosyl)-α/β-D-glucopyranose (isomaltose) or 6-O-(α-D-galactopyranosyl)-α/β-D-glucopyranose (melibiose) core. Carbohydrate Research. 2019, 473: 18-35.