Similar to genome, transcriptome or proteome, glycome is a set of complete glycans and glycoconjugates produced by cells or organisms under specific conditions. As opposed to amino acids in proteins or nucleotides in DNA, the monosaccharide units in sugar chains can be linked to each other in many different ways, so their biosynthesis lacks direct templates and standard mode. The structural diversity of the oligosaccharide branch, the added diversity of complex terminal saccharides, as well as various chemical modifications, such as phosphorylation, sulfation and acetylation, all further increase the structural diversity of glycans. These huge structural diversities enable glycans to encode a large amount of information, including cell signals, immune responses, and molecular recognition.
Identifying and analyzing the quantity, structure, and function of glycans is difficult. But in recent years, with the development of science and technology and scientific researchers' continuous efforts, this task has become easier. Currently, a variety of high resolution and high sensitivity methods are available, including capillary electrophoresis (CE), high-performance liquid chromatography (HPLC) and lectin microarrays, etc.
Fig 1. General Glycomic Strategies (Cummings, R.D.; et al. 2013)
The focus of glycan profiling is to capture the basic information of the target glycan in a high-throughput, rapid and highly sensitive manner. It is divided into two types: N-glycan and O-glycan profiling.
In order to fully understand the biological properties of glycans, the primary sequence and structure need to be clarified. We provide many highly specific exoglycosidases for sequence analysis.
We provide numerous glycan-related microarrays, such as lectin/ bacterial glycan/ customized microarrays, which can be used in genome research, as well as other biological and medical fields.
CD BioGlyco is a first-class glycomics analysis company, and our countless successful cases have won us a lot of praise in the international arena. We hope to start the exploration of glycobiology with you.
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