GOS is also known as oligogalactosyllactose, oligogalactose, and oligolactose. Because of the configuration of its glycosidic bonds, GOS largely resists hydrolysis by salivary and intestinal digestive enzymes. GOS belongs to prebiotics, and supports the natural defenses of the human body via the gut microflora, indirectly by increasing the number of bacteria in the gut and inhibiting the binding or survival of Escherichia coli, Salmonella typhimurium, and Clostridia. GOS positively influences the immune system indirectly through the production of antimicrobial substances, reducing the proliferation of pathogenic bacteria.
Fig.1 Physiological benefits of GOS. (e Souza, et al., 2022)
Based on advanced solutions, CD BioGlyco provides various Oligosaccharide Analysis Services to meet the specific requirements of our global clients. Currently, the most widely used method for the determination of total GOS content in food products is high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). We use HPAEC-PAD to detect the content and purity of GOS in various functional foods referring to the AOAC official method 2001.02. This is the only fully validated official method for the determination of GOS in food samples. The working principle is the comparison of a control solution (treated with the deactivated enzyme) with one that has been treated and hydrolyzed with the active enzyme β-galactosidase from Aspergillus oryzae.
Fig.2 Determination techniques of the GOS. (CD BioGlyco)
We use high-performance liquid chromatography (HPLC) to separate, identify, and quantify GOS at very low concentrations or dissolved in solvents. Gas chromatography coupled with mass spectrometry (GC-MS) and HPLC with fluorescence detector (HPLC-FLD) are also commonly used separation and characterization techniques, providing a basis for the study of structural information of polysaccharides. These techniques are applied to the quality assessment of new functional ingredients in herbal medicines and functional foods.
CD BioGlyco focuses on innovation and the application of technology to provide our clients with the most reliable GOS analysis service. We continuously track and research the latest technologies and strive to provide state-of-the-art solutions. If you are interested in our services, please contact us for more details.