CD BioGlyco is a world-leading company that can provide the best solutions for global customers in the field of microarrays. We have successfully completed many scientific research and non-clinical research projects to ensure that we provide the best products and services to customers all over the world.

Plant Glycomics Research and Microarray

Most plant polysaccharides, especially those derived from plant cell walls, are complex heteropolymers, and even purified ones contain a series of smaller oligosaccharide substructures (potential epitopes). The heterogeneity and diversity of glycoforms in the polysaccharide category affect the development of this resource and hinders the research on the role of some polysaccharide epitopes in crop physiology and pathology. A detailed understanding of the structure, function, interaction, and occurrence of plant glycans is essential for understanding its complex contribution to plant life and fully utilizing its commercial potential. The microarray and enzyme-linked immunosorbent assay (ELISA) composed of partially defined polysaccharide samples have the characteristics of high-throughput screening.

Fig. 1 Identification of epitopes of monoclonal antibodies targeting plant cell wall polysaccharides using a plant polysaccharide array. (Ruprecht, et al., 2022)

Phyto-glycan Microarray Service

Our services can help research and develop plant cell wall polysaccharide resources, characterize the structure of plant polysaccharides, and develop structural epitopes bound by plant polysaccharides and mAbs that determine the direction of cell wall glycans. Currently, the mainstream microarray-based glycan screening method is Comprehensive Microarray Polymer Profiling (CoMPP), which is very suitable for tracking glycan changes in complex biological systems and providing a global overview of cell wall composition.

• Using a miniaturized microarray platform to screen multiple samples, the spot signal on the microarray can be measured to provide semi-quantitative data about the appearance of glycan epitopes.
• There are currently more than 200 monoclonal antibodies directed against most types of plant cell wall polysaccharides.
• A series of well-defined monoclonal antibodies can be used as molecular probes to infer the accuracy of the presence or abundance of polysaccharides.

Fig 2. Flow chart of CD BioGlyco's plant cell wall glycan array screening service.

The use of synthetic plant carbohydrate microarrays can develop plant cell wall glycan epitope information, help the analysis of polysaccharide structure, and provide the required information for the specific detection of many complex molecular structures in plant cell wall polysaccharides.

Workflow

• Sample processing: We use an optimized extraction procedure to separate multiple cell wall polysaccharides. Each extracted fraction is then purified, concentrated, and quality-controlled to ensure its suitability for microarray printing.
• Microarray construction: Sample polysaccharide extracts are spotted onto solid surfaces using advanced printing technology. The density and layout of the arrays are optimized to enable high-throughput screening.
• Incubation and detection: The microarrays are incubated with carefully selected monoclonal antibody probes targeting known plant cell wall polysaccharide epitopes and scanned for detection. These antibodies serve as particular probes that bind exclusively to corresponding polysaccharide structures, such as xyloglucan oligosaccharides, galactan oligosaccharides, etc.
• Data analysis: After scanning with the scanner and obtaining images, we analyze the raw data through a rigorous bioinformatics processing workflow to interpret the binding profiles, identify specific polysaccharide epitope information, and provide useful insights into the compositional characteristics of plant cell walls.

Applications

• Understanding cell wall polysaccharide epitopes in cell lines aids in analyzing the cell wall composition of different plant varieties, guiding the development of plant biomass for use in biofuel and biochemical production.
• Microarray analysis of polysaccharide epitopes aids in studying changes in cell wall polysaccharide composition at different stages of plant development or under environmental stress, revealing the roles of these polysaccharides in growth regulation and stress adaptation.
• Microarray analysis of polysaccharide epitopes to analyze cell wall polysaccharide composition provides reliable support for exploring the use of this plant in the preparation of hydrogels or other biomaterials.

Advantages

• Our researchers are continuously optimizing microarray printing and detection. This ensures that we are constantly improving our capabilities to develop microarrays that meet the needs of various research applications.
• We carefully screen individual monoclonal antibodies to simultaneously screen a large number of plant polysaccharide epitopes, which helps to quickly and comprehensively characterize the cell wall composition of multiple samples, with a throughput far exceeding that of traditional methods.
• The high specificity of the monoclonal antibodies used ensures that binding signals accurately reflect cell wall polysaccharide epitopes.

Publication Data

Frequently Asked Questions

CD BioGlyco provides high-quality plant cell wall glycan array screening services. We have a well-equipped experimental platform, a large and professional team of scientists, and we are committed to cooperating with researchers from all over the world to meet the needs of our customers. Customers can contact our employees directly and provide timely feedback on their queries. If you are interested in our one-stop solution service, please contact us for more detailed information.

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