Mammalian Glycomics Microarray Scanning Service
CD BioGlyco is a world-leading company that can provide the best solutions for global customers in the field of microarrays. We have successfully completed many scientific research and non-clinical research projects to ensure that we provide the best products and services to customers all over the world.
Glycan microarrays have greatly changed the specific analysis methods of animal carbohydrate-binding proteins. The information obtained by this method can simultaneously clarify the biology mediated by the corresponding sugar group and decode the sugar group's own information. Advances in glycan array technology are parallel to complementary technologies used to interrogate the structure and function of glycans. These cross-used technologies include chemical glycobiology and analytical glycomics, which together facilitate rapid progress in the decoding of the information content of the glycan group. Arrays of oligosaccharide and polysaccharide molecules are used to more fully study these interactions. Array products assembled with glycan libraries for mammalian glycogen diversity have been successfully used to analyze mammalian biology. For example, host-pathogen interaction, immune recognition of glycans related to vaccine production, and analysis of glycan-binding proteins of cancer antigens.
Our service is founded on a robust and highly sensitive microarray platform that immobilizes a broad library of well-defined mammalian glycans on a solid surface, such as a glass slide. This creates an organized, miniaturized format for simultaneous analysis of hundreds of glycan-binding interactions. Our specialized arrays feature a diverse panel of biologically relevant glycan structures, enabling a comprehensive survey of a sample's binding specificities. We utilize advanced detection systems, including high-resolution confocal laser scanners, to precisely quantify the binding signals from each glycan spot. This method is highly efficient and requires only a minimal amount of sample, making it a valuable tool for precious or limited biological materials.
Our service strategy is a comprehensive microarray polymer analysis (CoMPP) technology derived from the analysis of plant sugars. This is a mature semi-quantitative method for analyzing the presence of carbohydrate epitopes in samples. Collect animal samples to extract the sugar components in the sample, and then use a series of anti-glycan antibody detection to obtain a series of bioinformatics data composed of the extract, and quantify the signal intensity.
| Sample Pretreatment | The strategy of continuous fractional extraction is adopted to extract the polysaccharide components of the sample; |
| Microarray Printing | Design and print out the microarray of the extracted polysaccharide components of the sample; |
| Microarray Hybridization | Use a set of anti-glycan antibodies as probes to incubate with the printed microarray; |
| Color Reaction | Incubate with a specific secondary antibody to develop the color with a chromogenic agent; |
| Scanning | Use a microarray scanner to capture signals and produce data; |
| Data Processing and Analysis | Determine the fluorescence ratio of the hybridization signal of the test sample and the reference sample, and obtain the relative copy number information. |
DOI.: 10.1186/s13027-018-0197-2
Journal: Frontiers in Marine Science
Published: 2017
IF: 3.0
Results: This research adapted the comprehensive microarray polymer profiling technique—originally used for plant/algal cell walls—to screen diverse marine animals for fucoidan epitopes typically found in brown algae. Using monoclonal antibodies (BAM1–BAM4) against algal fucoidan, the authors identified BAM1 and BAM4 epitopes in six species across three phyla (Porifera, Mollusca, Chordata), including the boring sponge Cliona celata and the tunicate Ciona intestinalis. Spatial analysis in C. celata revealed these epitopes were localized exclusively on internal chamber surfaces (spongocoel), suggesting association with extracellular matrices involved in filter-feeding. The study demonstrates that high-throughput glycan microarrays can detect cross-kingdom polysaccharide epitopes, revealing unexpected structural similarities between algal and animal glycans. This approach opens avenues for exploring glycan evolution, horizontal gene transfer, and bioprospecting bioactive marine polysaccharides.
Mammalian glycomics microarray scanning service decodes functional glycan signatures across human cell surfaces—revealing critical targets in cancer, infection, and immune regulation. To extend this analytical precision into microbial recognition mechanisms, we offer Microbial Carbohydrates Services that characterize pathogen-associated molecular patterns (PAMPs) through:
CD BioGlyco provides mammalian glycomics characterization services. We have a well-equipped experimental platform, a large and professional team of scientists, and we are committed to cooperating with researchers from all over the world to meet the needs of our customers. Customers can contact our employees directly and provide timely feedback on their queries. If you are interested in our one-stop solution service, please contact us for more detailed information.
Reference
