Detection of Glycolytic Metabolites in Cancer Cells
An important feature of cancer glucose metabolism is the upregulation of glycolysis, which is manifested by an increase in glucose uptake and glycolytic metabolites. Detecting the production of glycolytic metabolites can understand the metabolic changes at each step in the glycolysis process of cancer cells, and help to find clinical diagnostic markers for potential targets of cancer therapy. CD BioGlyco can provide detection services for metabolites in the whole process of glycolysis from glucose uptake to lactate production.
What We Do
Based on our MOPCGM platform, we can provide liquid chromatography detection of all metabolites in the glycolytic metabolic pathway.
We provide detection of single metabolites in the glycolytic pathway. The specific metabolites detected are as follows, including but not limited to:
- Glucose uptake assay. The isotope-labeled glucose analog 2-DG exists in cells as 2-DG6P because 2-DG is not affected by other enzymes in the cell. 2-DG is metabolized to 2-DG6P in cells, and NADPH is generated by enzymatic oxidation. NADPH is detected by a color probe, and glucose uptake is detected by a microplate reader. Fluorescently labeled 2-NBDG and capillary probes accumulated by cell species represent cellular uptake of glucose. FDG-PET imaging quantifies glucose in cancer cells.
- Glucose consumption assay. Glucohexokinase catalyzes the production of glucose 6-phosphate from glucose, resulting in β-nicotinamide adenine dinucleotide, with absorbance detected at 340 nm. Glucose is oxidized by glucose oxidase to form gluconic acid and hydrogen peroxide. Hydrogen peroxide reacts with horseradish peroxidase, resulting in red quinone imine, and absorbance is measured at 530 nm. Glucose dehydrogenase generates NADH from glucose and NAD+, and the presence of NADH enables the luciferin precursor reductase substrate to eventually generate a light signal.
- Extracellular acidification rate assay. Glucose, rotenone/antimycin A compound, and 2-deoxyglucose were added in sequence to detect the extracellular acidification rate.
- We have a library of high-quality standards containing metabolites from the entire glycolysis process. It can help customers detect all glycolytic metabolites at one time.
- We provide all detection services for metabolites in the glycolysis pathway and individual detection services for a certain metabolite to meet all the needs of customers.
- Screening potential drug targets by analyzing the effect of different treatments on the glycolytic capacity of cancer cells.
- The changes in the glycolytic capacity of cancer cells provide ideas for the identification of sensitive populations for specific treatment methods.
CD BioGlyco provides liquid chromatography analysis of all metabolites in the glycolytic pathway of cancer cells and colorimetric and fluorescence detection of single products. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.
This service is for Research Use Only, not intended for any clinical use.