Due to the important role of cerebrosides in physiological and pathological processes, CD BioGlyco provides analysis services of cerebrosides in various samples for global clients. If you have a method development requirement for the cerebroside, please feel free to contact us.
Glycolipids are biomolecules linked to hydrophobic lipids by glycosidic bonds. Glycosphingolipids are glycolipids composed of sphingosine or ceramides whose lipid moieties and sugar chains are diverse and highly heterogeneous. Glycosphingolipids include cerebrosides, gangliosides, globosides, glycosphingolipids, and glycophosphatidylinositols. Cerebrosides, also known as monosaccharylceramides, are composed of a ceramide backbone attached to neutral/non-acidic monosaccharide "head" groups such as galactose and glucose. The ceramides linked by galactose are known as galactocerebrosides, which are the predominant glycosphingolipids in brain tissue. Galactocerebrosides are distributed in all neural tissues and serve as markers of oligodentinal cell function. Glucose-linked ceramides are called glucocerebrosides, which are the predominant glycosphingolipids in brain tissue and are distributed in all neural tissues. Glucocerebrosides are also a major component of skin lipids and contribute to the skin's permeability to water. The synthesis of cerebrosides is accomplished through the process of glucose or galactose from nucleotide adhesion or direct transfer to ceramide molecules. The process occurs in the membranes of the endoplasmic reticulum and Golgi apparatus in eukaryotic cells.
Fig.1 Cerebroside-involved metabolism of sphingolipid. (Romero-Guevara, et al., 2015)
Cerebroside exists in animals, plants, bacteria, and fungi. It not only participates in the biosynthesis of disacylceramide, but also has important biological functions. Galactocerebroside is essential for myelin sheath and glucocerebroside plays a role in the skin's water-permeable barrier function. In addition, studies have found that abnormal metabolism of cerebroside is associated with many diseases. For example, Krabbe disease is a degenerative disease caused by the accumulation of galactocerebroside due to the dysfunction of lysosomal deposition, which in turn affects the myelin sheath and the white matter of the nervous system.
Due to the important role of cerebrosides in physiological and pathological processes, it is necessary to quantity accurately and sensitively cerebrosides in samples. Currently, thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and mass spectrometry (MS) have been reported for the analysis of cerebrosides. At CD BioGlyco, we have developed a complete solution for sphingolipid analysis. We provide high-sensitivity, high-resolution, high-precision, and reproducible cerebroside analysis services for our global clients by Q Exactive Orbitrap-MS. Our analysis range is 1) Total length from 30 to 46; 2) Total double bond from 0 to 2; 3) Total hydroxyl group from 2 to 4. We also provide method development services according to your specific needs.
In addition, we provide high-quality cerebroside products for global clients, including but not limited to:
CD BioGlyco has been focusing on lipidomics research for many years and has developed a complete one-stop solution for lipidomics analysis. We are willing to share our knowledge and experience in this field. If you are interested in our services and products, please contact us for more details without any hesitation.