Lipidomics Analysis Services
At CD BioGlyco, your lipidomics research is in the hands of scientists with decades of experience in lipidomics analysis. We provide targeted and untargeted lipidomics services to meet your specific project needs.
Lipids and Lipidomic Profiling
Lipids are amongst the most important organic compounds in living organisms, where they serve as building blocks for cellular membranes as well as energy storage and signaling molecules.
Lipidomic profiling is the systematic analysis and identification of lipids in organisms, tissues, or cells and the molecules with which they interact. In addition, the structure and function of lipids can also be understood, to reveal the relationship between lipid metabolism and the physiological and pathological processes of cells, organs, and even the body. The contents of lipidomics research mainly include analysis and identification of lipids and their metabolites, lipid function and metabolic regulation, lipid metabolism pathways, and networks. Lipidomics is the science of the large-scale determination of individual lipid species, and the underlying analytical technology that is used to identify and quantify the lipidome is generally mass spectrometry (MS).
Fig.1 The lipidomics workflow. (Züllig, et al., 2020)
Analytical Techniques of Lipidomics
The techniques of lipidomics research mainly include sample preparation, extraction, separation, data analysis, and identification of lipids and the corresponding bioinformatics techniques. At CD BioGlyco, we provide a wide range of lipidomics analyses. We mainly provide lipidomic analysis services based on mass spectrometry technology. The lipid analysis strategy based on mass spectrometry mainly includes liquid chromatography-mass spectrometry and shotgun lipidomics.
- Liquid chromatography-mass spectrometry
After using general liquid-liquid methods for extraction of most lipids within a sample, they are separated chromatographically using high-performance liquid chromatography (HPLC) or ultra-performance liquid chromatography (UPLC) to simultaneously detect individual molecular species and identify changes in these lipids between samples. Detection and identification of lipid species are achieved using high-resolution mass spectrometry (HRMS) and tandem mass spectrometry (MS/MS).
- Shotgun lipidomics
The shotgun lipidomics technique strategy usually adopts direct injection, and the lipid extract is directly analyzed and identified without chromatographic separation.
Lipidomics Analysis Services
The lipidomics analysis services we provide include but are not limited to fatty acyl analysis, glycerolipid analysis, phospholipids analysis, sterol lipids analysis, and sphingolipid analysis.
Oxidized lipids are lipid metabolites that add oxygen to lipid molecules and can be produced by enzymatic and non-enzymatic reactions. It plays a very important regulatory role in the life activities of organisms. The analysis of oxidized lipids can help to understand these life activities. CD BioGlyco detects them and analyzes oxidation products in free fatty acids by liquid chromatography-mass spectrometry.
Excessive neutral lipids (NLs) are closely associated with various human diseases such as diabetes. Therefore, qualitative and quantitative analysis of NLs may be helpful for related health research. CD BioGlyco has always adhered to the customer-centric principle and provides a one-stop neutral lipids analysis service by HPLC.
Advantages of Us
- Expert consultation on best practices for sample collection, pre-shipment processing, and storage to preserve sample integrity.
- Advanced technology: Ultra-high resolution, ultra-high sensitivity, fast scanning speed, and high-throughput mass spectrometry analysis.
- Strict quality control: From sample processing, quality evaluation to machine testing, every step is strictly controlled to ensure the accuracy of the results.
- Timely updates, interactive real-time data review, and comprehensive reporting with strict confidentiality.
CD BioGlyco aspires to become the trusted solution provider of your first choice. Our advanced technologies and highly experienced staffs are committed to advancing your program and reducing the overall development timeline. Our professional research teams provide customers with high-quality lipidomics analysis according to your needs. For further details, please don't hesitate to contact us.
Reference:
- Züllig, T.; et al. Lipidomics from sample preparation to data analysis: a primer. Analytical and bioanalytical chemistry. 2020, 412(10): 2191-2209.
Insights into Crystallization of Neuronal Nicotinic α4β2 Receptor in Polarized Lipid Matrices
The study utilized our UPLC-ESI-MS/MS-based Lipid Analysis Service to characterize phospholipid profiles, enabling high-resolution lipid identification and accurate data acquisition.
