With many years of experience in glycoengineering, CD BioGlyco has solved various shortcomings of the expression systems and brought customers high-quality cell lines to produce glycoproteins. We have confidence to be your essential research assistant in the field of glycobiology.
The Pichia pastoris expression system has the characteristics of fast growth, easy culture, simple genetic manipulation, and can perform glycosylation of proteins, It has been recognized as a powerful tool for large-scale protein expression and is broadly applied in bioengineering. Yeast first generates Man9GlcNAc2 sugar chain under the action of 1,6 mannose transferase encoded by och1 gene, which triggers the continuous addition of multiple mannoses on the sugar chain, forming a high mannose chain Man10-100GlcNAc2 which is different from mammalian cells.
The high mannose chain severely affects the structure and reduces the biological activity of the protein. At the same time, the protein containing the high mannose chain can cause an immune response in the body. Because the yeast expression system has the disadvantage of excessive glycosylation modification, it cannot replace the mammalian cell expression system in the production of various glycoproteins. Therefore, solving the problem of the high mannose chain is the core step of P. pastoris glycosylation engineering.
The therapeutic glycoprotein produced by the current glycoengineered P. pastoris platform has shown the same folding, stability and efficacy in vitro and in vivo as its counterpart produced from CHO cells in preclinical models. It is evident that P. pastoris provides a cost-effective alternative platform for the production of therapeutic glycoproteins.
Fig 1. Overview of yeast and mammalian N-linked glycosylation and the strategy used for glyco-engineered P. pastoris expression system (Vervecken, W.; et al. 2014)
CD BioGlyco applies genetic engineering technology to modify the glycosylation pathway of P. pastoris. By knocking out the och1 gene and introducing mannosidase, we constructed a glycoengineered P. pastoris expression system, which overcomes the excessive glycosylation and the final glycoprotein produced has a uniform low-molecular sugar chain. This new expression system can produce glycoproteins with glycosylation properties similar to mammalian systems. It has the advantages of short production cycle, low cost, high yield, easy operation and conducive to industrialized scale-up production, and will have a significant impact on protein expression and biopharmaceuticals.
The services we provide include but are not limited to the following processes
CD BioGlyco provides glyco-engineered Pichia pastoris express system that can be used for large-scale industrial production and one-stop services from gene synthesis to protein purification.
Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.