Peptidoglycan Purification

CD BioGlyco's experienced researchers have generated numerous successful cases in the field of peptidoglycan purification and structural characterization. We have confidence to be your essential research assistant in the field of glycobiology.

Background

The bacterial cell wall comprises peptidoglycan (PG), a large molecular network of sugar chains cross-linked by short peptides. The basic structure of PG contains a carbohydrate backbone with alternating units of N-acetylglucosamine (GlcNAc) and N-acetylmuramic acid, in which N-acetylmuramic acid residues are cross-linked with peptides. The glycan chains are cross-linked by short peptide bridges for a strong network structure, and their composition may vary depending on the type of bacteria.

The bacterial cell wall is very important for the cell growth and division process. Under the pressure of several atmospheres, the cell wall can maintain the mechanical integrity of cells. It gives the bacterial cell wall rigidity, protects bacteria from environmental damage, defines the shape of bacteria, and acts as an anchor for proteins and other polymers. The importance of peptidoglycan in bacterial physiology lies in its use as an antibiotic target, and promotes genetic, structural and cell biology research, so that it can be firmly assembled during growth and division. However, extensive research is still needed to fully characterize the detailed structure of peptidoglycan and the chemical composition of bacterial cell walls. Before performing detailed structural characterization, high-quality and highly selective purification of peptidoglycan is required.

Fig 1. Schematic structure of the cell wall in Gram-positive and Gram-negative bacteriaFig 1. Schematic structure of the cell wall in Gram-positive and Gram-negative bacteria (Pazos, M.; Katharina Peters, K. 2019)

Services

CD BioGlyco provides UPLC and HPLC-based peptidoglycan purification services.

Compared with traditional techniques (such as amino acid analysis or paper chromatography), the advantages of using HPLC to analyze the chemical composition of PG include higher resolution, shorter analysis time, and accurate quantification. UPLC has higher speed and sensitivity compared to HPLC, and can achieve faster flow rates, thereby reducing run time. Our services can provide quantitative information about all the chemical substances in samples. We can also provide a one-stop service from purification to subsequent structural characterization.

Applications

  • Bacterial cell wall structure and integrity research
  • Structure and function research of peptidoglycan
  • Microbiology and agriculture research

Advantages of Us

  • High selectivity and sensitivity
  • Able to handle a variety of sample types
  • One-stop service
  • Cost-effective

Our experienced researchers can provide customers with high-quality peptidoglycan purification services to facilitate subsequent peptidoglycan structure analysis experiments. CD BioGlyco will continue to improve our standards to meet customers' glycobiology research needs in a comprehensive and systematic way.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

References:

  1. Bertsche, U.; Gust, A.A. Peptidoglycan isolation and binding studies with LysM-Type pattern recognition receptors. Methods in molecular biology. 2017.
  2. Pazos, M.; Peters, K. Peptidoglycan. Sub-cellular biochemistry. 2019, 92.
This service is for Research Use Only, not intended for any clinical use.

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