Nanoparticles for Glycopeptide Enrichment
Nanoparticles are widely used for the isolation and separation of glycopeptides due to their nano dimensionality, tightly controlled size, and distinctive optical and electrochemical properties. CD BioGlyco has developed a variety of particle-based materials based on their properties to provide scientific assistance to customers in the field of glycoproteomics.
Nanoparticles
Nanoparticles that are widely used in assembling chips for biomolecule enrichment have the following characteristics: 1) nanoparticles with various size distributions and morphologies can be synthesized; 2) nanoparticles can be modified and diversified by a variety of reagents for specific applications; 3) they display unique optical and electrochemical properties.
In general, nanomaterials use various interactions between different functional groups bonded on the surface of various carriers and glycopeptides to directly enrich glycopeptides in the sample solution, which shortens the analysis time and reduces sample loss. This strategy not only simplifies the experimental procedure but also improves the sensitivity of the material for the detection of target glycopeptide chains, which is superior for the analysis of trace glycoproteins/glycopeptides. Moreover, the large surface area and easy surface modification of the nanomaterials facilitate the capture of the target glycopeptide chains.
Our Strategies
CD BioGlyco has established various glycoprotein/glycopeptide separation and enrichment approaches based on detonation nanodiamond (DND), magnetic nanoparticles (MNPs), polymer nanoparticles, gold nanoparticles(GNPs), SiO nanoparticles as the carriers. We provide our clients with several efficient strategies for glycopeptide enrichment, including but not limited to:
- We typically employ core-shell structured nanoparticles to enrich glycopeptides in complex samples, modifying the outer layers with various organic or inorganic reagents to identify biomarkers of interest.
- We fabricate aminophenyl boronic acid-functionalized magnetic nanoparticles for selective capture of glycopeptides from mixtures containing non-glycomolecules.
- We use a layer-by-layer method to synthesize MNPs, which are coated by multilayered polysaccharide shells and we employed these hydrophilic materials to selectively enrich glycopeptides from biological samples.
- We have developed a novel gold nanoparticle functionalized with ultra-small hydrazide groups capable of isolating 90% of the glycopeptides from complex biological samples.
Your Professional Glycopeptide Enrichment Partner
- We employ a diverse range of engineered nanoparticles, including magnetic nanoparticles, silica-based nanoparticles, and polymeric nanoparticles, each functionalized with specific chemistries tailored for optimal glycan recognition and binding.
- These functionalized nanoparticles exhibit high affinity for various glycan structures, enabling selective capture of glycopeptides from complex biological matrices such as serum, plasma, cell lysates, and tissue homogenates.
- Our approach ensures that glycopeptides are enriched with high purity, even in protein-rich biological matrices, by minimizing non-specific protein adsorption and ensuring the targeted interaction of our functionalized nanoparticles with the glycan moieties.
Workflow
Applications
- Our service is instrumental in biomarker discovery for diseases such as cancer, inflammatory disorders, and neurodegenerative diseases, where altered glycosylation plays a crucial role.
- Our service facilitates a deeper understanding of glycosylation's role in cellular processes, cell-cell interactions, and developmental biology.
- Our services can be used to analyze and evaluate glycosylated drugs, ensuring quality, efficacy, and safety.
Advantages of Us
- Our proprietary nanoparticle technology delivers unparalleled enrichment efficiency, typically achieving a 5-10 fold increase in glycopeptide signal intensity compared to conventional methods.
- The technology used by our company is highly adaptable, allowing for customization for specific glycan structures or broad glycosylation patterns.
- Our optimized process not only significantly improves the signal-to-noise ratio but can also be used for the enrichment and analysis of low-abundance glycopeptides.
Publication Data
Technology: Energy dispersive X-ray (EDX), Fourier transform infrared spectroscopy (FT-IR), Transmission electron microscopy (TEM), Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)
Journal: Scientific Reports
Published: 2017
IF: 3.8
Results: This document reports a polymeric hydrophilic ionic liquid modified magnetic (Fe3O4@MPS@PMAC) nanoparticles synthesized by one-step reflux precipitation polymerization. The nanoparticle exhibits high selectivity for N-linked glycopeptides through hydrophilic interactions and electrostatic interactions, with high detection sensitivity, large binding capacity, and satisfactory enrichment recovery rate (approximately 82%). Researchers successfully identified 1,274 N-glycans in the glycoprotein analysis of HeLa cell exosomes, demonstrating their potential in glycoproteinomics and exosome research.
Fig.1 Synthesis route of Fe3O4@MPS@PMAC nanoparticles. (Jiao, et al., 2017)
Frequently Asked Questions
CD BioGlyco continues to work on the development and synthesis of new affinity materials to solve the problem of isolation and enrichment of low abundance glycoproteins/glycopeptides from complex biological samples. If you are interested in our services, please feel free to contact us for further inquiries.
Associated Services
CD BioGlyco offers a range of complementary services to further support your glycoproteomics research and achieve your overall goals.
Reference
- Jiao, F.; et al. Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides. Scientific Reports. 2017, 7(1): 6984. (Open Access)
