More research focuses on the structure and function of glycolipids, and the important prerequisite for studying glycolipids is to obtain high-purity glycolipid samples. Our scientists are experienced in glycolipid separation and profiling, and we have the confidence to be your essential research assistant in the field of glycobiology.

Background

Carbohydrates mainly exist in the form of sugar complexes in organisms, including glycoproteins, proteoglycans and glycolipids. The basic structure of glycolipids is formed by connecting the hemiacetal hydroxyl groups of sugars with lipids through glycosidic bonds. It is a type of amphiphilic molecule that exists widely in organisms.

Glycolipids are often used as cell surface markers. It is also an important component of cell surface antigens. After some normal cells become cancerous, the surface glycolipids composition changes significantly, some isolated cancer cell characteristic antigens have also been shown to be glycolipids. The glycolipids on the cell surface are also receptors for many extracellular physiologically active substances, participating in cell recognition and information transmission. Over the years, a variety of glycolipid profiling strategies have been developed to directly promote the development of glycobiology, and these need to be based on advanced glycolipid separation technology.

Fig 1. Glycolipids with sterol, ceramide or diacylglycerol as hydrophobic backbone (Cortés-Sánchez, A. J.; et al.)

Services

At CD BioGlyco, we use the following methods to perform glycolipid separation.

• Thin-layer chromatography (TLC) and the newly developed high-performance thin-layer chromatography (HPTLC) have the characteristics of easy handling, robustness, automation, low-cost and reasonable separation capabilities. We also provide TLC with densitometric scanning and MS.
• Ion Mobility Separation (IMS) has many advantages, such as being able to distinguish between isomers and no special requirements for samples, etc. We also provide the combined use of IMS MS, MS/MS, desorption electrospray ionization (DESI) and mass spectrometry imaging (MSI).
• The separation of glycolipids by mass spectrometry combined with liquid chromatography has the characteristics of rapidness, high sensitivity, reproducibility and high resolution. We provide normal phase LC (NP-LC), reverse phase LC (RP-LC), high performance liquid chromatography and ultra-high performance liquid chromatography (HPLC and UHPLC) to achieve better separation of glycolipids.

Applications

• Cell-cell interactions and signal transduction research
• Discovery of cell surface biomarkers
• Function and structure research of glycolipids
• Pharmaceutical and biomedical research

Advantages of Us

• Combination of multiple advanced technology platforms
• Provide customized experimental design according to needs
• High resolution and consistency
• Cost-effective

CD BioGlyco provides a variety of advanced technology platforms for customers to solve various problems encountered in the separation and purification of glycolipids, and finally provide high-purity glycolipids for subsequent experimental use.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

References:

1. Sarbu, M.; Zamfir, A.D. Modern separation techniques coupled to high performance mass spectrometry for glycolipid analysis. Electrophoresis. 2018, 39.
2. Cortés-Sánchez, A. J.; et al. Biological activity of glycolipids produced by microorganisms: New trends and possible therapeutic alternatives. Microbiological Research. 2013, 168: 22-32.