Nanotechnologies have contributed to the realization of glycopeptide enrichment methods, especially from complex samples in preparation for MS characterization. CD BioGlyco uses nanomaterials with various properties and morphologies such as nanoparticles, mesoporous materials and nanotubes to help customers improve the sensitivity and specificity of glycopeptide separation.

Carbon Nanotubes

Carbon nanotubes (CNTs) are allotropes of carbon and possess cylindrical nanostructures. They have excellent mechanical, thermal, electrical and optical properties, and are used individually or in combination to provide keen sensors or on the other hand multifunctional materials. In addition, they have high angle proportions that are well suited for long and durable detection. Therefore, CNTs are widely used in the electronics industry, optical industry and other fields of materials science and technology.

Functionalized CNTs are applied in biology as biosensing platforms and biocomposites, and both applications enhanced glycan signal detection. The former (electrochemical biosensor) has shown high sensitivity, selectivity and fast response in analyzing mannose in human lung cancer cells. Previous studies have developed a new lectin-based electrochemical biosensor constructed from functionalized multi-walled CNTs for competitive determination of glycan expression on living cells. With the selection of more lectins with the biosensor advancement, this strategy has promising applications in the assay of other glycans on living cancer cells.

Our Strategies

To meet the scientific requirements of our customers, CD BioGlyco has established a variety of highly specific glycopeptide enrichment methods based on CNTs materials. The strategies we offer include but are not limited to:

• We developed a strategy for the stepwise synthesis of boronic acid-functionalized magnetic carbon nanotubes (MCNTs) that allows for highly specific glycopeptide enrichment even in the presence of large amounts of interfering non-glycopeptides.
• A novel lectin-based functionalized multi-walled CNTs electrochemical biosensor was constructed to capture glycans from living cells.
• Thiomannosyl dimer was synthesized to construct CNT/thirosine/Au-S-mannose. This biocomposite utilizes CNTs as carriers to load large amounts of thionine to generate electrochemical signals and AuNPs to anchor the thiomannosyl dimer.

Workflow

CD BioGlyco's CNTs for glycopeptide enrichment service follow a meticulously optimized workflow to ensure high efficiency and data quality.

• Sample digestion: The process begins with the enzymatic digestion of complex protein mixtures from various biological sources. Typically, proteins are denatured, reduced, and alkylated before enzymatic cleavage (e.g., with trypsin) to generate peptides, including glycopeptides, suitable for enrichment.
• CNT incubation and binding: Our researchers achieve optimal binding efficiency by precisely controlling incubation time, temperature, and buffer conditions.
• Washing and purification: Following binding, a series of stringent washing steps are performed to remove unbound non-glycopeptides and other interfering substances. This purification is critical for reducing background noise and enhancing the signal-to-noise ratio in downstream analysis.
• Glycopeptide elution: The enriched glycopeptides are then efficiently eluted from the CNTs using a precisely formulated elution buffer.
• MS Preparation: The eluted glycopeptide fractions are then prepared for MS.

Applications

• Our services can be used to elucidate the complex roles of protein glycosylation in various biological processes, such as cell signaling, cell-cell recognition, immune regulation, and protein folding.
• Our services can be used to study pathological changes in glycoproteins in disease states to gain a deeper understanding of disease progression and identify potential therapeutic targets.
• Our services can be used to study host-pathogen interactions affected by glycosylation and identify potential targets for antiviral or antibacterial treatments.

Advantages of Us

• Our proprietary CNT functionalization technology ensures exceptional specificity and dramatically enhanced sensitivity for glycopeptide capture. This translates into a significantly lower detection limit for glycopeptides, allowing for the identification of previously undetectable low-abundance species.
• The high surface area and unique electronic properties of CNTs contribute to superior binding capacity and rapid kinetics, streamlining the enrichment process and reducing overall analysis time.
• Our robust protocols ensure high reproducibility and minimal sample loss, crucial for quantitative glycoproteomics.

Frequently Asked Questions

CD BioGlyco offers a variety of prospective selective enrichment platforms for precise glycoproteomics analysis, and we will provide the most suitable strategy and service according to our client's needs and submit a detailed evaluation report. Please contact us for further consultation.

Associated Services

To provide a holistic solution for your glycoproteomic investigations, CD BioGlyco offers a range of complementary services that integrate seamlessly with our CNTs for glycopeptide enrichment:

• Custom Nanomaterial Synthesis and Functionalization
• Glycomic Characterization Service
• Glycoprotein Structure Analysis