The hyperglycemic clamp assay and the hyper-insulinemic euglycemic clamp assay are the gold standards for assessing the sensitivity of β cells to insulin and the effects of insulin, respectively. They are usually used to evaluate the glucose metabolism status of mice. Both these two experimental techniques detect the metabolic process of glucose and the function of insulin in tumor-bearing mice under a stable blood sugar state through the variable infusion of glucose or insulin. CD BioGlyco provides hyperglycemic clamp and hyper-insulinemic euglycemic clamp assays services to assess the ability of insulin secretion and β cell function in awake tumor-bearing mice.
The hyperglycemic and hyper-insulinemic euglycemic clamp assays are designed based on a glucose-insulin negative feedback system. The hyperglycemic clamp assay to assess pancreatic response to hyperglycemia. When the body's blood sugar rises, insulin secretion increases and the body lowers blood sugar by increasing the uptake, storage, and utilization of glucose. When the body's blood sugar drops, insulin secretion decreases, glucagon secretion increases, and the body raise blood sugar by promoting metabolism and accelerating glycogenolysis and gluconeogenesis pathways.
Fig. 1 The hyperglycemic clamp assays profile (Gerst F, et al., 2018)
These assays provide an assessment of insulin secretion capacity, pancreatic function, and β cell function in awake tumor-bearing mice.
CD BioGlyco provides hyperglycemic clamp and hyper-insulinemic euglycemic clamp assays services. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.