Porous graphitized carbon (PGC) is an essential tool in the chromatograph armory. CD BioGlyco is experienced in performing various glycan separation techniques and accumulated many years of experience in developing efficient methods of PGC chromatography. We look forward to cooperating with you in the field of glycobiology and becoming your indispensable research assistant.

Introduction

Due to the diversity of isomeric structures of glycoproteins, the analysis of enzymatically released glycans is challenging. Liquid chromatography-mass spectrometry (LC-MS) is a commonly used quantitative glycan analysis technique, which performs glycan separation and characterization. Although numerous reversed and normal stationary phases have been used for the glycans separation, no single chromatographic technique can completely separate glycans.

PGC has attracted much attention due to the advantages of good chemical stability, strong retention of polar compounds, and better peak patterns for acidic and basic compounds. HILIC and PGC chromatography are often carried out before mass spectrometry (MS) analysis. HILIC can sufficiently resolve many N-glycans, and PGC chromatography can separate the structure of underivatized N-glycan isomers based on dispersive interactions and a polar retention effect on graphite. The decomposed N-glycan isomers are then analyzed by mass spectrometry to obtain the mass of glycan, and the glycan was dissociated in the process of tandem mass spectrometry (MS/MS) to generate fragment ions showing the specific structure information of isomers.

Fig.1 Separation of a reduced trisaccharide O-glycan derived from k-casein using PGC-LC-MS. (Cho, 2020)

Services

At CD BioGlyco, the coupling of PGC column and high-resolution mass spectrometer can be used for isomeric separation of permethylated glycans. The services we offer include but are not limited to:

• Anomeric and isomeric separation of O-glycans.
• Separation of free oligosaccharides and glycosphingolipid (GLS) glycans. We analyze different sources of free oligosaccharides by using C18 and PGC columns, and separate GSL-glycans from different cancer cell lines or biological fluids from patients with various diseases by PGC-LC.
• Separation of permethylated N-glycans.

Applications

• Research on antibody-based therapeutics
• Functional research of O-glycans in cancer and other diseases
• Examination of potential glycolipid-based cancer biomarkers

Advantages of Us

• Constantly updating methods
• Responsible attitude
• Open and respectful communication
• Customer-oriented service

With many years of experience in developing PGC chromatography methods, CD BioGlyco has successfully applied PGC-LC-MS to characterize released glycans from complex samples. We are also very flexible in implementing existing methods to meet researchers' needs. If you are interested in our services, please contact us for more detailed information.

Reference:

1. Cho, B.G.; et al. Separation of permethylated O-glycans, free oligosaccharides, and glycosphingolipid-glycans using porous graphitized carbon (PGC) column. Metabolites. 2020, 10(11):433.