Many studies have revealed the association between haptoglobin (Hp) and various infections and diseases, such as cancer, diabetes and cardiovascular diseases. CD BioGlyco is committed to developing rapid and robust methods for the detection of Hp, providing powerful tools for clinical diagnosis.

Hp

Hp is an acute-phase protein and any inflammatory process (infection, burns, extreme stress, allergies, severe crush injuries, etc.) may increase plasma haptoglobin levels. Hp is often used as a predictor of human diseases such as ovarian cancer, diabetes, certain neurological and cardiovascular diseases. In the clinical setting, Hp detection is commonly used to diagnose and monitor hemolytic anemia. In intravascular hemolysis, free hemoglobin is released into the circulation and therefore Hp binds to the hemoglobin. This causes a decrease in Hp levels.

Hp is also a predicting diagnostic biomarker commonly used to predict animal diseases such as mastitis, which is a recurrent disease in the dairy industry. The spread of pathogens causing mastitis and the long-term decline in milk production and quality have caused major economic challenges to the global dairy industry. The detection of HP is helpful for the early diagnosis of mastitis, which may have a positive impact on the individual health of animals.

Fig.1 Schematic illustration of the CL bioassay concept for Hp detection. (Fatima, 2019)

Our Strategies

CD BioGlyco has developed multiple rapid and sensitive sensor-based assays for measuring Hp concentrations in mastitis milk. The Strategies we provide include but are not limited to:

• We use a number of analytical methods that can complement and validate each other including lectin blot, mass spectrometry (MS) and lectin antibody ELISA to assess the aberrant glycosylation of Hp in cancer.
• We have developed a label-free chemiluminescence bioassay based on magnetite nanoparticles (MNP) for sensitive Hp detection by utilizing the specific interactions of hemoglobin-modified MNPs.
• We employ an immunosensor for the detection of Hp concentration in milk samples by immobilizing anti-bovine Hp antibodies on a gold electrode with gold nanoparticles prepared on self-assembled L-cysteine layers, and there was an agreement between the results obtained by this method and ELISA.

Applications

• Early bio-diagnostic detection of mastitis
• Effects on individual animal health
• Development novel and accurate technology for early diagnosis of diseases
• Discovery of innovatory drugs on target proteins or specific sites

Advantages of Us

• Simple, rapid and cost-effective procedure
• Good stability and reproducibility
• Well-trained staff
• Advanced instrument platform

CD BioGlyco has accumulated extensive experience in glyco-biomarker Hp detection. We provide our clients with high-quality services at competitive prices and within a short time frame. With our efficient strategies, customers could accelerate their scientific research. If you are interested in our services, please contact us for further information.

Reference

1. Fatima, A.; Basu, A. Label free quantitation of immunoglobulin G using the stagnant cap hydrodynamic retardation effect detector (SHRED). IEEE SENSORS. 2019, 1-4.